For the following amino acids: Isoleucine and Tyrosinea. Draw its complete protonic equilibria. Indicate the net charge of each form and encircle the zwitterionic form. Provide labels (A, B, C, etc.) for each form. b. Calculate the IpH of the amino acid. c. At what pH range(s) it can act as a buffer? d. What will be the predominant form(s) of the amino acid at pH (i) 1.5; (ii) 3.0; (iii) 4.5; (iv) 6.0; (v) 7.5 and (vi) 9.0? Table 1: pka values of the 20 common amino acids.amino acidalaninearginineaspartateasparaginecysteineglutamateglutamineglycinehistidineisoleucineleucinelysinemethioninephenylalanineprolineserinethreoninetryptophantyrosinevalinepka1 (-CO₂H)2.342.171.882.021.962.192.172.341.822.362.362.182.282.201.992.212.112.382.202.32pk, valuesPK82 (-NH₂)9.699.049.608.8010.289.679.139.609.179.689.608.959.219.1310.969.159.629.399.119.62pKar (R group)12.483.658.184.256.0010.5310.07

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

For the following amino acids: Isoleucine and Tyrosine

a. Draw its complete protonic equilibria. Indicate the net charge of each form and
encircle the zwitterionic form. Provide labels (A, B, C, etc.) for each form.
b. Calculate the IpH of the amino acid.
c. At what pH range(s) it can act as a buffer?
d. What will be the predominant form(s) of the amino acid at pH (i) 1.5; (ii) 3.0; (iii) 4.5;
(iv) 6.0; (v) 7.5 and (vi) 9.0?