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What is the most common sterilization technique used in laboratories?

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter4: The Prokaryotes

Section: Chapter Questions

Problem 4UTM

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Concept explainers

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."

Question

1. What is the most common sterilization technique used in laboratories?

2. List at least 5 procedures of the aseptic technique and describe its uses.

3. Why is Gram stain one of the most important and widely used stains in bacteriology?

4. Explain the Gram staining technique in chronological order. Indicate the reagent used and the time of usage on each reagent.

Definition Definition Technique that uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.

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INSTRUCTIONS: Read each sentence carefully Encircle the biological techniques that are being described. 1. Itis also known as sterile technique. Aseptic technique Disinfection Answer: 2. It is described as the complete removal of microorganisms found in a specimen or sample. sterilization Disinfection Answer: 3. It is described as the elimination of pathogens that reduce the microbial contamination. sterilization Disinfection Answer: 4. This is described as the elimination of life processes in a specimen. Killing Fixation Answer: 5. Clearing is also known as Dehydration Dealcoholization Answer: