What is the function of DNA gyrase? O a prevents the two complementary template strands from bonding back to each other O b.reduces the torsional strain (knotting) that builds up ahead of the replication fork as a result of unwinding Oc connects Okazaki fragments Od umvinds the double helix at the replication fork

What is the function of DNA gyrase? O a prevents the two complementary template strands from bonding back to each other O b.reduces the torsional strain (knotting) that builds up ahead of the replication fork as a result of unwinding Oc connects Okazaki fragments Od umvinds the double helix at the replication fork

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 17P

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What is the function of DNA gyrase?
O a prevents the two complementary template strands from bonding back to each other
Ob.reduces the torsional strain (knotting) that builds up ahead of the replication fork as a result of unwinding
Oc connects Okazaki fragments
Od umvinds the double helix at the replication fork

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Replication involves a period of time during which DNA is particularly susceptible to the introduction of mutations. If nucleotides can be incorporated into DNA at a rate of 20 nucleotides/second and the human genome contains 3 billion nucleotides, how long will replication take? How is this time reduced so that replication can take place in a few hours?
Make the complementary strand for the following DNA template and label both strands as 5 to 3 or 3 to 5 (P = phosphate in the diagram). Draw an arrow showing the direction of synthesis of the new strand. How many hydrogen bonds are in this double strand of DNA? template: PAGGCTCGOH new strand:
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Origin of replication [ Choose ] [Choose] Codon Complementary pairs with cytosine in a DNA molecule The site of protein synthesis Ribozyme This molecule can begin the synthesis of a polymer of nucleotides without a primer A short peice of lagging stand DNA Primase A reflection of redundancy in the genetic code RNA which exhibits enzymatic properties Okazaki Fragment The process of making RNA from DNA Helicase DNA polymerase Transcription Enzyme which lays down a segment of RNA on replicating DNA so that DNA nucleotides can polymerize A sequence of three nucletotides which codes for an amino acid Ribosome Point Mutation Chromosome Wobble Position The point on a stand of DNA where replication begins Mitochondria RNA polymerase [ Choose ] Guanine
Considering that DNA is synthesized in the 5' to 3' direction, which direction must DNA polymerase travel along the template strand: 5' to 3' or 3' to 5' ?
In DNA Replication, what is the function of Primase: works to connect discontinuous parts of DNA such as the nicks created by gyrase and the okazaki fragments O Synthesizes RNA on a DNA template to provide an available 3'-OH group to continue with DNA synthesis O binds at the origin and begins to unwind the helix O bind near the origin to maintain the complementary sequences of DNA from reforming the helical structure
vvnicn the following statements are correct about the repair of a DNA duplex containing the sequence below that is grown INE coli (select all that apply)? Strand A Strand B GATCTAGCCGGCATCCGAT CTAGATCGGACGTAGGCTA Methyl ✔A. MutH cleaves Strand A O B. DNA repair will result in the bold A in strand B being replaced with a C O C. DNA repair will result in the bold G in strand A being replaced with a T ✔ D. Defect will not be properly repaired in dam(-) E coli O E. The mammalian repair system would also correct the mismatch shown based on the methylation status of the DNA
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If primase makes an error and incorporates an incorrect base in the nucleic acid primer, then why would this not be a problem given the DNA replication mechanism? O Primase only copies end sequences that will be protected by telomers The lagging strand utilizes a helicase to prevent replication errors O Primase can be error proofed using a domain of DNA polymerase O The RNA primer sequence is removed and not used as a template
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During DNA replication, the function of RNA primers is to Group of answer choices serve as a binding site for DNA ligase separate the two strands of the double helix to open replication "bubbles" serve as starting points for DNA strand elongation by DNA polymerase in the 3' - 5' direction prevent new-separated strands of DNA from rejoining serve as starting points for DNA strand elongation in the 5' - 3' direction by DNA polymerase
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