What is the diffusion coefficient of a membrane-bound protein of molar mass 79,300 daltons density of 1.4 g-cm-3, 40 12.83e-9 xm²-s-1 What is the average distance traveled by this protein in 2.3 s? 401.709 t 37°C if the viscosity of the membrane is 1 poise (0.10 N-s-m-2)? Assume that this protein is an unhydrated, rigid sphere that has a

What is the diffusion coefficient of a membrane-bound protein of molar mass 79,300 daltons density of 1.4 g-cm-3, 40 12.83e-9 xm²-s-1 What is the average distance traveled by this protein in 2.3 s? 401.709 t 37°C if the viscosity of the membrane is 1 poise (0.10 N-s-m-2)? Assume that this protein is an unhydrated, rigid sphere that has a

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

A sample of a protein is analyzed by CE using a neutral-coated capillary with a total length of 25.0 cm and a distance to the detector of 22.0 cm. Using an applied voltage of 20.0 kV, the protein was detected at a migration time of 16.2 min. The diffusion coefficient for the protein is 2.4 x 10-7 cm?/s. (a) Calculate the apparent electrophoretic mobility of the protein. (b) Calculate the expected number of theoretical plates for the analysis.
A one-to-one protein (P)-ligand (L) complexation (P + L PL) has a dissociation equilibrium constant (Kd) value of 100 nM at 25°C, and the Kd remains the same at 37°C. 1) What is AS of binding at 25°C? Assume ACp of the binding is 0 over the temperature range. AS = 1.34E2 kJ/(mol*K) (note the unit!!) (sig. fig =3) 2) What is the concentration of the PL complex formed at equilibrium when you mix 0.20 uM (microM) of Protein and 1.0 uM of Ligand together at 37°C? PL at equilibrium = 8.1E-1 uM (note the unit!!) (sig. fig =2)
The following table shows experimental results of the glucose transport rate, mM/sec, following facilitated diffusion by glucose carrier proteins. (Recall: the starting conc. L represents glucose added to one side of the membrane; distilled water, omM of glucose was added to the other side of the membrane). The rate of glucose transport was 0.0031 mm/sec with 8mM of glucose (run number 4, highlighted); the rate decreased to 0.0017 mM/sec with 10mM of glucose (run 5, highlighted). Why was the rate of glucose transport slower when the concentration gradient was increased? Experiment Results Run Number Solute 1 1 2 2 3 33 4 4 5 6 6 Na Ch Glucose Na Ch Glucose Na Ch Glucose Nat Ch Glucose Na Ch Glucose Nat Cl Glucose Start Conc. L Start Conc. R (MM) (mM) 0.00 0.00 2.00 0.00 0.00 0.00 8.00 0.00 0.00 0.00 2.00 0.00 0.00 0.00 8.00 0.00 0.00 0.00 10.00 0.00 2.00 0.00 2.00 0.00 Carriers 500 500 500 500 700 700 700 700 100 100 700 700 Rate (mm/sec) 0.0000 0.0008 0.0000 0.0023 0.0000 0.0010…
Two proteins, A and B, bind to the same ligand, L, with the binding curvesshown below. What is the dissociation constant, Kd , for each protein? Which protein (A or B) has a greater affinity for ligand L?
19. 150 mCi of D2O is injected in your patient. 10% of D2O is excreted. The concentration of D2O in a plasma sample is found to be 0.4 mCi/100 ml. What is your patient' s Total Body Water in L? 20. the diffusion coefficient states that: A. the larger the molecular radius of the solute, the slower the rate of diffusion B. the more viscous the solution, the slower the rate of diffusion C. the smaller the molecular radius of the solute, the faster the rate of diffusion D. the more viscous the solution, the faster the rate of diffusion E. the larger the molecular radius of the solute, the faster the rate of diffusion 21 You are experimenting on diffusion rates in lab. Down the center of Beaker A is a semipermeable membrane. On one side of the membrane in Beaker A is a 100mM solution of CaCl2; on the other side is pure water.Down the center of Beaker B is a semipermeable membrane. On one side of the membrane in Beaker B is a 10mM solution…
bo Consider a uniport system where a carrier protein transports an uncharged substance A across a cell membrane. Suppose that at a certain ratio of [A] inside to [A] outside, the AG for the transport of substance A from outside the cell to the inside, Macmillan Leaming A outside → Ainside, is 10.7 kJ/mol at 25°C. What is the ratio of the concentration of substance A inside the cell to the concentration outside? [A]inside [A]outside Choose the true statement about the transport of A under the conditions described. Because AG is positive, the ratio [A] inside/[A]outside must be less than one. Increasing [A]outside will cause AG for movement of Aoutside to Ainside to become a larger positive number. Movement of Ainside to Aoutside will be spontaneous. Decreasing the concentration of the uniport protein in the membrane will cause AG to become a smaller positive number.
If crystals of deoxy Hb are exposed to air, they break. What does this imply about the 4o structure of deoxy Hb and oxy Hb and predict which subunits junctions are most affected in the change from deoxyHb to oxyHb?
How can I calculate the unknown protein please
A solution containing 3.58 x 1023 molecules/m3 of protein in water is separated from pure water by a membrane 3.20 μm thick. The diffusion coefficient of the protein through the membrane is 7.15 x 10-18 m2/s. On average, how many molecules cross 0.0240 μm2 of this membrane each second? [Answer as a positive number with 3 sig digits, but do not enter units with your answer]
Calculate θ for a certain protein-ligand pair when the ligand concentration = 1 M and the Kd = 1 X 10-15 M.
When the potato cube is surrounded by a hypotonic solution what change in mass occurs in the potato cells?
Shown below are cells (colored) that were recently placed into a beaker containing a clear solution For each scenario, indicate whether movement of the molecule into the cell will occur using facilitated diffusion or active transport. А. B. 125mM 20mM fructose glucose 85MM 35mM fructose glucose OA= facilitated diffusion; B= facilitated diffusion OA= active transport; B= active transport O A= active transport; B= facilitated diffusion A= facilitated diffusion; B= active transport