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What are the materials and methods to achieve identification code (not the actual identification code) and the method to achieve the unknown identity since the "unknown organism" is identified using a numerical code from the bioMerieux catalog/APIweb that lists each reference species with its 7 digit code. Gram stain of the isolate to be identified, the oxidase test , the API 20E strip used and the reagents added .

What are the materials and methods to achieve identification code (not the actual identification code) and the method to achieve the unknown identity since the "unknown organism" is identified using a numerical code from the bioMerieux catalog/APIweb that lists each reference species with its 7 digit code. Gram stain of the isolate to be identified, the oxidase test , the API 20E strip used and the reagents added .

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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What are the materials and methods to achieve identification code (not the actual identification code) and the method to achieve the unknown identity since the "unknown organism" is identified using a numerical code from the bioMerieux catalog/APIweb that lists each reference species with its 7 digit code. Gram stain of the isolate to be identified, the oxidase test , the API 20E strip used and the reagents added .
### API® 20 E System for Bacterial Identification **Overview:** The API® 20 E is a standardized system for the identification of Enterobacteriaceae and other non-fastidious, Gram-negative bacteria. It consists of 20 microtubes containing dehydrated substrates that enable various biochemical tests. **Components:** - **Microtubes Panel:** - The panel includes the following tests: ONPG, ADH, LDC, ODC, CIT, H2S, URE, TDA, IND, VP, GEL, GLU, MAN, INO, SOR, RHA, SAC, MEL, AMY, ARA. Each microtube contains a specific substrate. - **Color Indicators:** - The microtubes display different colors after bacterial incubation, indicating positive or negative results for each biochemical test. For instance, ONPG turns yellow when positive. **Procedure:** 1. Bacterial suspension is prepared using the test kit instructions. 2. The suspension is added to the microtubes. 3. After incubation, reagents are added if necessary to develop reactions. 4. Results are interpreted based on color changes. **Diagram Explanation:** - The top section shows a strip of 20 microtubes containing different substrates. - The bottom section (data sheet) has circles corresponding to each tube with markers (+/-) to indicate positive or negative outcomes. **Application:** - Used for bacterial identification in microbiological laboratories. - Facilitates rapid and reliable identification of various strains based on biochemical properties. **Created by:** - **BIOMÉRIEUX** - A company specializing in microbiological diagnostics. **Reference:** - REF: #2 - Date: 2023/10/12 This system is essential for researchers and healthcare professionals involved in microbial diagnostics and offers a reliable method for differentiating bacterial species based on biochemical characteristics.
Transcribed Image Text:### API® 20 E System for Bacterial Identification **Overview:** The API® 20 E is a standardized system for the identification of Enterobacteriaceae and other non-fastidious, Gram-negative bacteria. It consists of 20 microtubes containing dehydrated substrates that enable various biochemical tests. **Components:** - **Microtubes Panel:** - The panel includes the following tests: ONPG, ADH, LDC, ODC, CIT, H2S, URE, TDA, IND, VP, GEL, GLU, MAN, INO, SOR, RHA, SAC, MEL, AMY, ARA. Each microtube contains a specific substrate. - **Color Indicators:** - The microtubes display different colors after bacterial incubation, indicating positive or negative results for each biochemical test. For instance, ONPG turns yellow when positive. **Procedure:** 1. Bacterial suspension is prepared using the test kit instructions. 2. The suspension is added to the microtubes. 3. After incubation, reagents are added if necessary to develop reactions. 4. Results are interpreted based on color changes. **Diagram Explanation:** - The top section shows a strip of 20 microtubes containing different substrates. - The bottom section (data sheet) has circles corresponding to each tube with markers (+/-) to indicate positive or negative outcomes. **Application:** - Used for bacterial identification in microbiological laboratories. - Facilitates rapid and reliable identification of various strains based on biochemical properties. **Created by:** - **BIOMÉRIEUX** - A company specializing in microbiological diagnostics. **Reference:** - REF: #2 - Date: 2023/10/12 This system is essential for researchers and healthcare professionals involved in microbial diagnostics and offers a reliable method for differentiating bacterial species based on biochemical characteristics.
This image displays an API 20E test strip, which is used for the identification of Enterobacteriaceae and other Gram-negative bacteria. The strip contains 20 miniature biochemical tests, each in its own well, designed to determine the metabolic capabilities of the bacteria. Here's a description of the components from left to right: 1. **ONPG**: Tests for β-galactosidase activity. 2. **ADH**: Tests for arginine dihydrolase. 3. **LDC**: Tests for lysine decarboxylase. 4. **ODC**: Tests for ornithine decarboxylase. 5. **CIT**: Tests for citrate utilization. 6. **H2S**: Tests for hydrogen sulfide production. 7. **URE**: Tests for urease activity. 8. **TDA**: Tests for tryptophan deaminase. 9. **IND**: Tests for indole production. 10. **VP**: Tests for Voges-Proskauer reaction. 11. **GEL**: Tests for gelatinase activity, as indicated by the dark, possibly gel-like appearance. Following these are sugar fermentation tests (GLU to ARA), which assess the ability of the organism to ferment various sugars, producing acid or gas as a byproduct, resulting in a color change: - **GLU**: Glucose - **MAN**: Mannitol - **INO**: Inositol - **SOR**: Sorbitol - **RHA**: Rhamnose - **SAC**: Sucrose - **MEL**: Melibiose - **AMY**: Amygdalin - **ARA**: Arabinose Each of these compartments shows a color change, indicating positive or negative results that help identify the bacterial species.
Transcribed Image Text:This image displays an API 20E test strip, which is used for the identification of Enterobacteriaceae and other Gram-negative bacteria. The strip contains 20 miniature biochemical tests, each in its own well, designed to determine the metabolic capabilities of the bacteria. Here's a description of the components from left to right: 1. **ONPG**: Tests for β-galactosidase activity. 2. **ADH**: Tests for arginine dihydrolase. 3. **LDC**: Tests for lysine decarboxylase. 4. **ODC**: Tests for ornithine decarboxylase. 5. **CIT**: Tests for citrate utilization. 6. **H2S**: Tests for hydrogen sulfide production. 7. **URE**: Tests for urease activity. 8. **TDA**: Tests for tryptophan deaminase. 9. **IND**: Tests for indole production. 10. **VP**: Tests for Voges-Proskauer reaction. 11. **GEL**: Tests for gelatinase activity, as indicated by the dark, possibly gel-like appearance. Following these are sugar fermentation tests (GLU to ARA), which assess the ability of the organism to ferment various sugars, producing acid or gas as a byproduct, resulting in a color change: - **GLU**: Glucose - **MAN**: Mannitol - **INO**: Inositol - **SOR**: Sorbitol - **RHA**: Rhamnose - **SAC**: Sucrose - **MEL**: Melibiose - **AMY**: Amygdalin - **ARA**: Arabinose Each of these compartments shows a color change, indicating positive or negative results that help identify the bacterial species.
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