Solve the identity of an unknown bacterial specimen by creating a dichotomous key. Categories: Gram stain, Mannitol salt agar,MacConkey agar, Catalase, Oxidase, Casein hydrolysis, Nitrate reduction, Citrate test, Urease test, Sim Medium Streptoccocus ID: 1) Bile Esculin Test 2) Bacitracin Test 3) Blood Agar Plate NaCl Broth Staphylococcus ID: 1) Mannitol Salt Agar 2) DNA Hydrolysis 3) Coagulase Test 4) Novobiocin Test Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Solve the identity of an unknown bacterial specimen by creating a dichotomous key.
Categories:
Streptoccocus ID:
1) Bile Esculin Test
2) Bacitracin Test
3) Blood Agar Plate
NaCl Broth
Staphylococcus ID:
1) Mannitol Salt Agar
2) DNA Hydrolysis
3) Coagulase Test
4) Novobiocin Test
Possible Organisms
- Alcaligenes faecalis
- Enterobacter aerogenes
- Enterococcus faecalis
- Escherichia coli
- Proteus vulgaris
- Pseudomonas aeruginosa
- Salmonella arizoniae
- Staphylococcus aureus
- Staphylococcus epidermidis
- Staphylococcus saprophyticus
- Streptococcusbovis
- Streptococcus pyogenes
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