Explain the activities associated with the isolation of Bacillus subtilis organsim from mixed culture to pure culture. Include the relevant information( type of inoculation, source of bacteria etc)?  Also the isolation of the organism from the broth containing a fresh mixture of 2 or more bacteria was obtained after incubation of nutreint agar plate.

Q: For Staphylococcus aureus, indicate which tests need to be done & what media you need. Fill in box…

A: Staphylococcus aureus is a spherical-shaped cocci that belongs to the family of Firmicutes. It is…

Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…

A: A dichotomous key is a tool designed by scientists to determine the identity of the organism. Here,…

Q: What is the correct shape of the unknown bacterium growing on your MacConkey agar?

A: Macconkey Agar This agar includes bile salts, which are found in the gut. Bile salts help in…

Q: which agent was most effective against the fecal coliform that was tested? how does this agent…

A: Fecal coliforms are a kind of bacteria present in the intestines of warm-blooded animals such as…

Q: Is Endospores stain test a efficient test for unknown intestinal bacteria?

A: Staining provides valuable information as to bacterial morphology, gram reaction, and presence of…

Q: Describe how you would execute this first stage from the point you are handed the nutrient broth…

A: For isolation of any culture, it needs to be incubated in broth to obtain full fledged turbidity or…

Q: When innoculate the bacteria noticed yellow colonies, is that staphylococcus aureus or…

A: It is required to identify the yellow colonies seen after inoculation of the bacteria is…

Q: while working with mannitol salt agar you notice the growth of staphylococci however other…

A: Mannitol Salt Agar is used as a selective and differential medium for the identification and…

Q: Which of the following tests can be used to distinguish between group D streptococcus and other…

A: Group D is the classification of streptococcus bacteria. These are gram positive bacteria, have…

Q: Which type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus…

A: A culture medium, also called the growth medium, can be a liquid or solid medium that supports the…

Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…

A: The question asks about the test that can be used on the disease-causing organism below that can…

Q: Explain the activities associated with the isolation of Bacillus subtilis organsim from mixed…

A: Bacteria are single-celled microorganisms that are found in virtually every environment on Earth.…

Q: Explain why the media where the Staphylococcus aureus is plated has turned yellow but the media…

A: Mannitol Salt Agar (MSA) is a differential and selective medium. A member of the staphylococcus…

Q: МАС EMB Agar НЕА SSA XLD Agar Escherichia coli Klebsiella pneumoniae Citrobacter freundii Serratia…

A:

Q: Explain the reason for the effect on mass spectroscopy method of diagnosis by culture conditions.

A: Mass Spectrometry is an analytical method. It is used to quantify known materials to identify…

Q: List 3 species of bacteria that can spoil milk and indicate how they react to the gram staining…

A: Spoilage of milk and milk products results from growth of fermentative bacteria when storage…

Q: mention any three fluids used in cleaning lense in the microbiology laboratory

A: A laboratory tool called a microscope is used to look at things that are too tiny to be seen with…

Q: Which of the following is not meant to reduce contamination of yoru sample? avoiding hand contact…

A: A microbial culture is done to multiply the specific strains of the microorganisms under specific…

Q: Explain the significance of the Snyder agar medium and its differential nature as a means to detect…

A: Dental caries is also called tooth decay or cavity. It causes the formation of tiny holes in the…

Q: Indicate which are the laboratory conditions necessary to cultivate Clostridium bacteria in medium,…

A: A significant human pathogen that is a causative agent for many diseases like botulism, tetanus,…

Q: What method of bacterial measurement does this graph represent? Is it considered direct, or…

A: Here Bacterial growth is measured in terms of absorbance so, bacterial growth is measured with the…

Q: Which of these microbial structures is the most difficult to kill when attempting to sterilize…

A: Answer of the question given below...

Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…

A: Dichotomous Key:A ‘dichotomous key’ is a series of questions about characteristics of an organism,…

Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…

A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…

Q: Considering microbial identification methods, to which category would the following method belong…

A: Bacteria are identified routinely by morphological and biochemical tests, supplemented as needed by…

Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…

A: A dichotomous key is a tool made by the scientist to identify any living being based on its…

Q: Based on the growth you saw on your Lee’s Agar plate, what conclusions can you make about the…

A: Lee Agar is used to study the bacteria that can grow on yogurt. It is primarily used to count the…

Q: Did the yogurt congeal? Did obtain the expected bacteria on Lee’s agar? What factors may have led to…

A: Lee's agar is used specifically for differential enumeration of bacteria especially starter bacteria…

Q: Explanation of results in context of unknown Test Purpose Reagents Used Gram-stain Endospore…

A: Pseudomonas aeruginosa  It is an encapsulated, rod shaped bacteria which is responsible for the…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.