What are some of weaknesses of BLASTP searching and Conserved Domain searching for proteinannotation?Conserved Domain doesn't provide ALL the potential domains of a proteinBLASTP cannot assign function to proteinConserved Domain lacks capability for complete functional annotation.Conserved Domain only gives a portion of the whole protein

Bioinformatics is an interdisciplinary field of study which has diverse application in the field of Biology. Bioinformatics uses computer programming, mathematical modelling, statistics, computational analytical tools, algorithms, data science and software to to enable researchers to understand and analyze complex and large size of biological data, for example nucleic acid sequence, amino acid sequence, proteomics, genomics, protein structure, sequence similarity, protein structural and domain analysis, functional classification of different gene and protein families, etc. Protein sequence analysis by multiple sequence analysis tools have become of immense significance in the field of bioinformatics, medical research for construction of database and query. BLASTp is a multiple sequence alignment tool of NCBI which is majorly used to find out evolutionary relationship between different taxa or species by comparing their amino acid sequences of different proteins. The more the homology is found between two different protein sequences, the more closely related those species are. It can predict the time and the ancestor from where the species came from. evolution is a continuous process which is driven by genetic drift, mutation in the gene sequences resulting in generation of new polypeptide sequences. Molecular phylogenetic analysis investigates the changes in the linear amino acid sequence of the long chain of polypeptides, which are connected to each other by peptide bonds. This is the most simple structural conformations of proteins. But it can not give us complete information about the function of the proteins, or can not define what functional role it might play in different species. Bioinformatic analysis by conserved domain search is often used to find out specific similarity patterns among structurally or functionally similar amino acid sequences. Some structurally similar proteins share homology for a particular part of the protein sequences are often found to be under the same protein families. A certain region of amino acid homology can denote a structurally conserved domain which might play similar function across different species. Hence, conserved domain only gives information about a particular region of the whole protein. hence the appropriate options are: and