Vhat is needed from the cells for PCR?

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**What is needed from the cells for PCR?**

Polymerase Chain Reaction (PCR) requires specific components from cells to amplify DNA. Here’s what is necessary:

1. **DNA Template**: The original DNA extracted from the cells, which contains the target sequence to be amplified.

2. **Primers**: Short strands of nucleotides that provide a starting point for DNA synthesis. Primers are designed to match the starting and ending points of the target DNA sequence.

3. **DNA Polymerase**: An enzyme that synthesizes new DNA strands by adding nucleotides complementary to the template strand. Taq polymerase, a thermostable enzyme, is commonly used.

4. **Nucleotides (dNTPs)**: The building blocks of DNA, consisting of deoxynucleotide triphosphates (dATP, dCTP, dGTP, dTTP), which are added to the growing DNA strand during synthesis.

5. **Buffer Solution**: Maintains the optimal pH and salt conditions necessary for the DNA polymerase to function effectively.

By preparing these components, researchers can efficiently perform PCR to amplify the desired DNA regions for further study or applications.
Transcribed Image Text:**What is needed from the cells for PCR?** Polymerase Chain Reaction (PCR) requires specific components from cells to amplify DNA. Here’s what is necessary: 1. **DNA Template**: The original DNA extracted from the cells, which contains the target sequence to be amplified. 2. **Primers**: Short strands of nucleotides that provide a starting point for DNA synthesis. Primers are designed to match the starting and ending points of the target DNA sequence. 3. **DNA Polymerase**: An enzyme that synthesizes new DNA strands by adding nucleotides complementary to the template strand. Taq polymerase, a thermostable enzyme, is commonly used. 4. **Nucleotides (dNTPs)**: The building blocks of DNA, consisting of deoxynucleotide triphosphates (dATP, dCTP, dGTP, dTTP), which are added to the growing DNA strand during synthesis. 5. **Buffer Solution**: Maintains the optimal pH and salt conditions necessary for the DNA polymerase to function effectively. By preparing these components, researchers can efficiently perform PCR to amplify the desired DNA regions for further study or applications.
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