Vasopressin is a peptide anti-diuretic hormone that is important for maintaining serum osmolality. Vasopressin contains 9 amino acids in the sequence shown below. How many peptide bonds would be cleaved when vasopressin is treated with chymotrypsin? Chymotrypsin is an endopeptidase that hydrolyzes on the C-side of phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp). Vasopressin amino acid sequence: Cys - Tyr - Phe- Gln - Asn - Cys- Pro - Arg - Gly O peptide bonds cleaved no reaction with chymotrypsin O 1 peptide bond cleaved O 2 peptide bonds cleaved O 3 peptide bonds cleaved

Q: List all the amino acids you would expect to bind to a cation-exchange column at pH 2.

A: Chromatography is derived from two Greek words, chroma means colour and graphein means to write.…

Q: amino acid in the form of a "phenylthiohydantoin" (PTH-amino acid), are shown below. What is the…

A: Phenyl isothiocynate is the Edman reagent. It reacts with N-terminal amino acid to give phenyl…

Q: Consider the peptides Pro-Gin-Val-Phe-His-Asp-Cys and His-Gln-Pro-Cys-Asp-Phe-Val. How do these two…

A: Amino acids are biomolecules in which an amino group and a carboxyl group are linked to the same…

Q: When answering the questions below, please use the one-letter code for the amino acid, with NO…

A: Acid hydrolysis: Acid hydrolysis of any protein/polypeptide is being used to detect amino acids…

Q: (a) A solution containing these five proteins was adjusted to pH 7.0 and then applied to a…

A: Chromatography is a method of separation of specific compounds from the mixture of compounds. This…

Q: Draw a phosphorylated tyrosine molecule at physiological pH. Assume that the phosphate group is…

A: Tyrosine (Tyr) is an aromatic amino acid. It plays an important role in metabolism and the…

Q: The tripeptide +H3N-Leu-His-Glu-COO- has pKa  values of 2.2, 3.6, 6.0 and 9.1.  The  isoelectric…

A: Isoelectric point (pI) is the pH at which there is no net charge on the molecule. For a amino acid,…

Q: The OH group of the serine side chain (R-OH) can undergo dissociation to form R-O- and H+. However,…

A: The proteins are constituted of amino acids linked via peptide linkages.The side chains of amino…

Q: The pK₂ of the x-carboxyl group of serine is 2.21, and the pKa of its a-amino group is 9.15.…

A: pKa is the pH at which a weak acid is 50% dissociated. % ionisation of a weak acid = % ionisation of…

Q: Write the one letter code for the oligopeptide Ser-Cys-Ile-Glu-Asn-Cys-Glu and calculate the total…

A: On a logarithmic scale from 0 to 14, pH, or "potential of hydrogen," measures the acidity or…

Q: Lipoprotein particles (Figure 3.18) are spherical clusters of protein and lipid molecules that…

A: When it comes to transport molecules, lipoproteins are classified as composed of a mix of lipids and…

Q: Amino acid Cys has ionizable groups with pKa values 2.0, 8.0, and 10.0.  Determine the charge on the…

A: For cysteine Charge when pH is at 1.0? ____+ve_____        Charge when pH is at 9.0? ____-ve_____

Q: The following questions refer to the peptide with a sequence of: Asp-Ser-Lys-Asn-Glu a) Would you…

A: Amino acids are biomolecules that have an amino group and a carboxyl group linked to the same carbon…

Q: The following peptide that has been acetylated at the C-terminus: CH3C(O)-GEEGH-NH2 Plot the…

A: Titration is a very useful procedure to estimate the concentration of any unknown compound in a…

Q: Lactic Acid (shown below) is built up in the body during intense workouts as a result of glycolysis.…

A: The carboxylic acid group (COOH) of lactic acid has a pKa of 3.85. The physiological pH of our body…

Q: A tripeptide has the following sequence:    Glu-Trp-Arg What is the net charge of this tripeptide…

A: The given peptide is composed of three amino acids. Peptide composed of three amino acids is called…

Q: At a pH of 10, would you expect this peptide to be retained for a longer time within an anion…

A: Ion exchange chromatography separates molecules based on their charge difference. A cation exchange…

Q: Calculate the pl of the following Peptide

A: At extreme low pH, molecule is fully protonated as the pH increase more than pKa of an ionizable…

Q: Vasopressin, a nonapeptide hormone secreted by the pituitary gland, functions by stimulating the…

A: Hormones are molecules secreted at a very low quantity from the endocrine glands. There are…

Q: How could you make the zwitterion of aspartic acid more soluble in water?

A: A zwitterion is considered as a chemical compound, which has both charges positive as well as…

Q: Consider a hexapeptide of the sequence thr asn glu trp lys gln. After complete hydrolysis, which…

A: Asked : Amino acid which elutes first

Q: The subunits of hemoglobin and myoglobin share structural similarities. Some residues are…

A: Hemoglobin and myoglobin are two types of globular proteins that bind to oxygen. Hemoglobin is found…

Q: The anomer of a-D-gluсоруranose IS CH2OH он ÓH он CH:OH он он он он CH,OH он он

A: Glucose is the primary source of energy for humans and other organisms as well. Glucose contain four…

Q: Describe the amino acid illustrated here (Identify the following amino acid (at pH = 7.0):…

A: Amino acids are the organic molecule composed of amino group (NH2), an acidic carboxylic group…

Q: Describe the amino acid illustrated above (at pH = 7.0). (the following amino acid at pH = 7…

A: Amino acids are the organic molecule composed of amino group (NH2), an acidic carboxylic group…