Using Beer's law, calculate the molar absorptivity (M-1 cm-1) of PNP from the absorbance. The path length of the cuvette is 1 cm, and the stock concentration of PNP is 0.0100 mM. Convert abs/sec to M/sec using the molar absorptivity determined in the previous step. You will use Beer's law again here but will solve for concentration instead of molar absorptivity. Convert M/sec to mol/sec by multiplying by the total reaction volume (3.00 mL). Convert mol/sec to nmol/sec. Convert nmol/sec to nmol/min. This value represents the amount of PNP produced (nmol) by alkaline phosphatase per minute. A=ebc A=0.163 b=1cm c=0.01mM Total Volume=3mL

Using Beer's law, calculate the molar absorptivity (M-1 cm-1) of PNP from the absorbance. The path length of the cuvette is 1 cm, and the stock concentration of PNP is 0.0100 mM. Convert abs/sec to M/sec using the molar absorptivity determined in the previous step. You will use Beer's law again here but will solve for concentration instead of molar absorptivity. Convert M/sec to mol/sec by multiplying by the total reaction volume (3.00 mL). Convert mol/sec to nmol/sec. Convert nmol/sec to nmol/min. This value represents the amount of PNP produced (nmol) by alkaline phosphatase per minute. A=ebc A=0.163 b=1cm c=0.01mM Total Volume=3mL

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

Using Beer's law, calculate the molar
absorptivity (M-1 cm-1) of PNP from the
absorbance. The path length of the cuvette
is 1 cm, and the stock concentration of PNP
is 0.0100 mM. Convert abs/sec to M/sec
using the molar absorptivity determined in
the previous step. You will use Beer's law
again here but will solve for concentration
instead of molar absorptivity. Convert
M/sec to mol/sec by multiplying by the total
reaction volume (3.00 mL). Convert mol/sec
to nmol/sec. Convert nmol/sec to
nmol/min. This value represents the
amount of PNP produced (nmol) by alkaline
phosphatase per minute.
A=ebc
A=0.163
b=1cm
c=0.01mM
Total Volume=3mL

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