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Urease is the enzyme that catalyzes the hydrolysis of urea into carbamic acid which is then cleaved into carbon dioxide and ammonia. Increased level of this enzyme is associated with some diseases such as stomach cancer, urinary tract infections, and liver cirrhosis. Hence, a wide array of studies has been done to inhibit this enzyme such as extracts from plant sources. Using your knowledge in enzyme inhibition, determine the effect of a hexane extract from Cinammomum camphora, a member of plant species used in a large number of traditional medicines. The following data are available: Urea Concentration, mM Rate of reaction (mM/min) 10 20 30 40 60 80 100 Without plant extract 0.256 0.441 0.544 0.650 0.926 1.232 1.538 With plant extract 0.170 0.312 0.410 0.497 0.636 0.693 0.849 Answer the following: a. Give the linear regression parameters (slope, y-int, and r) of the Lineweaver-Burk plot in the presence and absence of the plant extract. Linear regression parameters Slope y-intercept Linearity constant Without the plant extract With the plant extract b. KM of the enzyme for the substrate in the absence and presence of the plant extract. Report answers in 2 decimal places. Round off your answer. KM (without the plant extract): KM (with the plant extract): C. Vmax of the reaction in the absence and presence of the plant extract. Report answers in 2 decimal places. Round off your answer. Vmax (without the plant extract): Vmax (with the plant extract): d. What is the type of inhibition exhibited by the plant extract? Basis:

Urease is the enzyme that catalyzes the hydrolysis of urea into carbamic acid which is then cleaved into carbon dioxide and ammonia. Increased level of this enzyme is associated with some diseases such as stomach cancer, urinary tract infections, and liver cirrhosis. Hence, a wide array of studies has been done to inhibit this enzyme such as extracts from plant sources. Using your knowledge in enzyme inhibition, determine the effect of a hexane extract from Cinammomum camphora, a member of plant species used in a large number of traditional medicines. The following data are available: Urea Concentration, mM Rate of reaction (mM/min) 10 20 30 40 60 80 100 Without plant extract 0.256 0.441 0.544 0.650 0.926 1.232 1.538 With plant extract 0.170 0.312 0.410 0.497 0.636 0.693 0.849 Answer the following: a. Give the linear regression parameters (slope, y-int, and r) of the Lineweaver-Burk plot in the presence and absence of the plant extract. Linear regression parameters Slope y-intercept Linearity constant Without the plant extract With the plant extract b. KM of the enzyme for the substrate in the absence and presence of the plant extract. Report answers in 2 decimal places. Round off your answer. KM (without the plant extract): KM (with the plant extract): C. Vmax of the reaction in the absence and presence of the plant extract. Report answers in 2 decimal places. Round off your answer. Vmax (without the plant extract): Vmax (with the plant extract): d. What is the type of inhibition exhibited by the plant extract? Basis:

Chemistry & Chemical Reactivity
Chemistry & Chemical Reactivity
10th Edition
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter14: Chemical Kinetics: The Rates Of Chemical Reactions
Section14.7: Reaction Mechanisms
Problem 1.2ACP
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Enzyme Inhibition

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Urease is the enzyme that catalyzes the hydrolysis of urea into carbamic acid which is then cleaved into carbon dioxide and ammonia. Increased level of this enzyme is associated with some diseases such as stomach cancer, urinary tract infections, and liver cirrhosis. Hence, a wide array of studies has been done to inhibit this enzyme such as extracts from plant sources. Using your knowledge in enzyme inhibition, determine the effect of a hexane extract from Cinammomum camphora, a member of plant species used in a large number of traditional medicines. The following data are available: Urea Concentration, mM Rate of reaction (mM/min) 10 20 30 40 60 80 100 Without plant extract 0.256 0.441 0.544 0.650 0.926 1.232 1.538 With plant extract 0.170 0.312 0.410 0.497 0.636 0.693 0.849 Answer the following: a. Give the linear regression parameters (slope, y-int, and r) of the Lineweaver-Burk plot in the presence and absence of the plant extract. Linear regression parameters Slope y-intercept Linearity constant Without the plant extract With the plant extract b. KM of the enzyme for the substrate in the absence and presence of the plant extract. Report answers in 2 decimal places. Round off your answer. KM (without the plant extract): KM (with the plant extract): C. Vmax of the reaction in the absence and presence of the plant extract. Report answers in 2 decimal places. Round off your answer. Vmax (without the plant extract): Vmax (with the plant extract): d. What is the type of inhibition exhibited by the plant extract? Basis:
Transcribed Image Text:Urease is the enzyme that catalyzes the hydrolysis of urea into carbamic acid which is then cleaved into carbon dioxide and ammonia. Increased level of this enzyme is associated with some diseases such as stomach cancer, urinary tract infections, and liver cirrhosis. Hence, a wide array of studies has been done to inhibit this enzyme such as extracts from plant sources. Using your knowledge in enzyme inhibition, determine the effect of a hexane extract from Cinammomum camphora, a member of plant species used in a large number of traditional medicines. The following data are available: Urea Concentration, mM Rate of reaction (mM/min) 10 20 30 40 60 80 100 Without plant extract 0.256 0.441 0.544 0.650 0.926 1.232 1.538 With plant extract 0.170 0.312 0.410 0.497 0.636 0.693 0.849 Answer the following: a. Give the linear regression parameters (slope, y-int, and r) of the Lineweaver-Burk plot in the presence and absence of the plant extract. Linear regression parameters Slope y-intercept Linearity constant Without the plant extract With the plant extract b. KM of the enzyme for the substrate in the absence and presence of the plant extract. Report answers in 2 decimal places. Round off your answer. KM (without the plant extract): KM (with the plant extract): C. Vmax of the reaction in the absence and presence of the plant extract. Report answers in 2 decimal places. Round off your answer. Vmax (without the plant extract): Vmax (with the plant extract): d. What is the type of inhibition exhibited by the plant extract? Basis:
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