Two ligands bind to the same site of a protein. However, when examined by ITC, for one of the ligands heat needs to be added to the system to maintain constant temperature, whereas for the other ligand, heat must be removed. Why is this the case?

Two ligands bind to the same site of a protein. However, when examined by ITC, for one of the ligands heat needs to be added to the system to maintain constant temperature, whereas for the other ligand, heat must be removed. Why is this the case?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Related questions

Q: disease. As such, a tron Type 2 diabetes arug metformin, which acts indirectly to inhibit…

A: Gluconeogenesis is the metabolic pathway that is responsible for the synthesis of glucose from…

Q: A protein X binds Ligand Y. The kon characterizing this binding is 1 x 106 M-1s-1, koff is 2 x 10-3…

A: The Lock and key model of protein ligand binding states that the binding sites of proteins are 100%…

Q: Rho, Rac, and Cdc42 are three different GTPases that regulate cytoskeletal dynamics. We've…

A: Introduction and overview GTPases are a large family of proteins that function as molecular…

Q: What is an allosteric site? How does an allosteric site differ from an enzyme's active site or a…

A: An allosteric site is a specialized regulatory site found on a protein. It exerts its influence by…

Q: Biolink 'Drugs and their Conformations' (a) Which drug is more likely to produce multiple…

A: Drugs and their conformation - drug conformation is the local arrangement of the atoms in a drug…

Q: 3CLpro is a cysteine protease, which are among the most common proteases in biochemistry, however…

A: The 3 dimensional conformation of a biomolecule is highly specific and vital to its function,…

Q: Let’s consider vmax. Recall that uncompetitive inhibitors bind to the enzyme only after the substate…

A: Enzymes are biocatalyst that performs specific chemical reaction within our body. They are…

Q: Phospholipase C targets the molecule O A. DAG for cleavage into two subunits. B. PIP2 C. PKA D. GTP

A: Phospholipase C (PLC) isozymes are essential signalling molecules. Extracellular stimuli, including…

Q: Cell signaling. The ER doctors gave a patient an injection of epinephrine to increase his heart…

A: Receptors are considered to be macromolecules that take part in signaling within and between cells.…

Q: A Allosteric site B Allosteric site C Allosteric site Allosteric effector Active site Allosteric…

A: A chemical known as an allosteric effector, often referred to as an allosteric regulator, binds to a…

Q: Troubleshooting gels. What effect will be observed on the

A: Polyacrylamide gel electrophoresis is used for the separation of protein molecules on the basis of…

Q: In hepatocytes, glucagon ilin inden release of select one, which leads to the activation of PKA. One…

A: Hepatocytes are responsible for regulating blood glucose level. When the blood glucose level…

Q: The Figure should illustrate the interactions made between the key components of (a) total and (b)…

A: There are two ways by which a ligand would bind to its receptor molecule namely specific & non…

Q: Biotransformation. Explain the process of enzyme induction. What are the benefits or down-falls of…

A: Enzymes are proteins that increase the rate of chemical reactions by lowering the activation energy…

Q: Need help, please.

A: Parameters such as Km and Vmax are used for comparing enzyme activities. If we know the initial rate…

Q: Reminder: Enzymology is foundational to biochemistry. You should review and be familiar with the…

A: A regulatory enzyme is an enzyme in a biochemical process that regulates the system's activity by…

Q: Need help, please.

A: MM kinetics describes the mathematical relation between initial rate of reaction(vo), maximum rate…

Q: In order to be activated, the [Select] to bind to [Select] [ Select ] [ Select ] [Select] N…

A: Introduction Cell signaling, also known as signal transduction, is the process by which cells…

Q: Look at the schematic illustration of a protein (3D conformation) belpw. If the highlighted SER…

A: Proteins are the biomolecules that are made up of Aminoacids joined by Peptide bond. Proteins exist…

Q: Which of the following subunits act as a GTPase, breaking GTP into GDP and a phosphate? OA.G gamma O…

A: Heterotrimeric G-proteins are composed of alpha, beta, and gamma subunits. The chemical abilities of…

Q: One form of DNA damage, methylation of adenosine residues, destabilizes them. At 37 °C, the tj/2 for…

A: DNA damage is a change in the underlying DNA structure and it may include chemical addition or…

Q: A 24-residue peptide called Pandinin 2, isolated from scorpion venom, was found to have both…

A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Q: The signal hypothesis, proposed by Gunter Blobel, was eventually shown to be: O correct, but it only…

A: correct, even more signals exist than just what Blobel studied

Q: control The product of a series of reactions acts as an inhibitor for an earlier reaction. Hormones…

A: Genetic control means involvement of genes in a particular process. Genes are small units of DNA…

Q: Immune-mediated myositis (IMM) is a muscle wasting disease that occurs in quarter horses with an…

A: Given information IMM or immune-mediated myositis is a muscle-wasting disorder. The mutation is…

Q: QUESTION 1 What are the functions of metal ions in catalysis? Select all that apply. O a. increasing…

A: The answer of this question is option d that is increased binding energy. * Metal ion help in…

Question

100%

Need help.

(a) Two ligands bind to the same site of a protein. However, when examined by ITC, for one of the ligands heat needs to be added to the system to maintain constant temperature, whereas for the other ligand, heat must be removed. Why is this the case?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Similar questions

Answer asap. Thank you.
P CO HI F. sum21.ex1.137 Accessibility Mo 日- Which of the following molecules generally possesses a complex shape that allows the molecule to function as tools and machinery that essentially do all the work within a living cell? 15. A. triglycerides B. proteins C. carbohydrate D. A and Bare correct. 16. Which of the following molecules is NOT involved in cell-to-cell signaling? A nroctaalandine Give %00% kidomi 56°F 近 Insert F8 F6 & %24 8. K 2 G oW N M
Need help, please. Not too sure why my answer is incorrect. Please show all steps/work.
Need help, please.
Do not give handwriting solution.
Small molecules are used as inhibitors of protein action - as drugs. They most often do this by blocking the active site within the protein. Potential drugs can be screened computationally to determine if they are strongly bound to the protein. Figure 1 shows a possible conformation of a candidate drug molecule, 4-bromo-2- carboxymethylamide-pyrrole (abbreviation: BCMAP) at the active site of a protein (abbreviation: PR). Figure 2 shows the full protein structure whilst figure 3 shows a known inhibitor of the protein at the site, overlayed with another calculated conformer of BCMAP. (a) Explain what types of interactions, both intermolecular and intramolecular, that a molecular mechanics forcefield must be able to describe in order to be able to accurately determine the geometry of BCMAP in the protein. Identify which interactions will be the most important to describe accurately. Figure 1.4-bromo-2-carboxymethylamide-pyrrole (BCMAP) (C, N, O, and Br atoms in yellow, blue, red, and…
Please help with these quickly.
Signal transduction pathway. Sketch a G protein in the active and inactive stages, and label its parts.
In Multi-Column Purification of rGFP. What happens to the protein amount, protein purity, and/or specific activity of a purification fraction if one of the three is changed? (i.e. understand the relationship between the three.)
1F. Please help me in detail. for molecular Mechanism of ATP versus GTP selectivity of adenylate kinase, Draw a reaction scheme that includes both the substrate and the inhibitor under study here. Be sure to label KI, Ks, kp