This question refers to experimentally determining a Michaelis- Menten curve for an enzyme. If you do not observe a plateau in reaction velocities for the range of substrate concentrations you have tested (i.e. the graph of initial reaction velocity versus substrate concentration is linear), which of the following changes would be most appropriate to try, in order to generate more informative data about Vmax and Km when repeating the assay? a. Test higher substrate concentrations. b. Test lower substrate concentrations. C. Use more concentrated enzyme. O d. Use more stop solution.

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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This question refers to experimentally determining a Michaelis-
Menten curve for an enzyme.
If you do not observe a plateau in reaction velocities for the range of
substrate concentrations you have tested (i.e. the graph of initial
reaction velocity versus substrate concentration is linear), which of the
following changes would be most appropriate to try, in order to
generate more informative data about Vmax and Km when repeating
the assay?
a.
Test higher substrate concentrations.
b. Test lower substrate concentrations.
C. Use more concentrated enzyme.
O d. Use more stop solution.
Transcribed Image Text:This question refers to experimentally determining a Michaelis- Menten curve for an enzyme. If you do not observe a plateau in reaction velocities for the range of substrate concentrations you have tested (i.e. the graph of initial reaction velocity versus substrate concentration is linear), which of the following changes would be most appropriate to try, in order to generate more informative data about Vmax and Km when repeating the assay? a. Test higher substrate concentrations. b. Test lower substrate concentrations. C. Use more concentrated enzyme. O d. Use more stop solution.
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