This question refers to experimentally determining a Michaelis- Menten curve for an enzyme. If you do not observe a plateau in reaction velocities for the range of substrate concentrations you have tested (i.e. the graph of initial reaction velocity versus substrate concentration is linear), which of the following changes would be most appropriate to try, in order to generate more informative data about Vmax and Km when repeating the assay? a. Test higher substrate concentrations. b. Test lower substrate concentrations. C. Use more concentrated enzyme. O d. Use more stop solution.
This question refers to experimentally determining a Michaelis- Menten curve for an enzyme. If you do not observe a plateau in reaction velocities for the range of substrate concentrations you have tested (i.e. the graph of initial reaction velocity versus substrate concentration is linear), which of the following changes would be most appropriate to try, in order to generate more informative data about Vmax and Km when repeating the assay? a. Test higher substrate concentrations. b. Test lower substrate concentrations. C. Use more concentrated enzyme. O d. Use more stop solution.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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