Need help, please. Please fill in the blanks to make these statements TRUE:In hepatocytes, glucagon signaling leads to release of select one ✓ which leads to the activation of PKA. One of the net effects of this signaling pathway is the select oneleads to the activation of select one ✓ which leads to the select one✓of select one, thus leading to select oneof this enzyme.CHECK ANSWERof FBase-2 vi✔ select onephosphorylationdephosphorylationnsulin signaling, on the other hand, Please fill in the blanks to make these statements TRUE:In hepatocytes, glucagon sinnaling loads to release of select one ✓ which leads to the activation of PKA. One of the net effects of this signaling pathway is the select oneleads to the activation c✔ select one which leads to the select oneof select one ✓ thus leading to select oneof this enzyme.,PKAPP1GSPFK1CHECK ANSWERof FBase-2 via select oneInsulin signaling, on the other hand,

Hepatocytes are responsible for regulating blood glucose level. When the blood glucose level decreases below the normal range, glucagon is released and hepatocytes in response start to release glucose into the blood. Hepatocytes can generate glucose via gluconeogenesis and glycogen breakdown. During this period, glycolysis is inhibited in the hepatocytes.When the blood glucose level increases beyond the normal range, insulin is released and hepatocytes in response start to absorb glucose from the blood and start either using them as metabolic fuel via glycolysis or stores it as glycogen.Glucagon binds to its G-Protein Coupled Receptors (GPCRs) in hepatocytes, which leads to the release of cAMP by adenylate kinase. cAMP goes onto activate Protein Kinase A (PKA). PKA then goes onto phosphorylate the PFK-2/FBPase-2 bifunctional protein. Phosphorylation leads to the activation of FBPase-2 activity of the enzyme. FBPase-2 converts fructose 2,6-bisphosphate to fructose 6-phosphate. This leads to stimulation of gluconeogenesis (by activating Fructose 1,6-bisphosphatase) and inhibition of glycolysis (by inhibiting PFK-1).Insulin signaling leads to the activation of the enzyme phosphoprotein phosphatase 1 (PP1). Phosphoprotein phosphatase 1 (PP1) goes onto dephosphorylate the bifunctional PFK-2/FBPase-2 protein. Dephosphorylation leads to activation of PFK-2 activity of the enzyme. PFK-2 synthesizes fructose 2,6-bisphosphate, which goes onto activate glycolysis (by activating PFK-1) and inhibit gluconeogenesis (by inhibiting Fructose 1,6-bisphosphatase).In hepatocytes, glucagon signaling leads to release of cAMP, which leads to the activation of PKA. One of the effects of this signaling pathway is the activation of FBPase-2 via phosphorylation. Insulin signaling on the other hand, leads to the activation of PP1, which leads to the dephosphorylation of PFK-2, thus leading to activation of this enzyme.