Topic: Transmittance and absorbance, lambert-beer law To determine the content of Cu in a sample, 0.3632 g are weighed, dissolved with 80 ml of acid and brought to a volume of 250 ml, the absorbance of the solution is measured together with a series of standards in cells of 1 cm and the data obtained were:  ppm Cu 0 2 4 10 12 Absorbance 0 0.082 0.164 0.410 0.440 The absorbance of the sample was 0.125. Calculate the mg of Cu in 100 g of sample

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UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or  ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.