The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected thefollowing data at 630 nm. The student also found that the absorbance of an FD&C Blue Dye #1 solution ofunknown concentration was 0.244. Use your Vernier Graphical Analysis to generate a standard curve from thisdata set, and use it to determine the concentration of the unknown solution. (Report your final answer to 3 sigfigs)Concentrationof Blue Dye (M)Absorbance@ 630 nm6.35x10-71.23x10-63.84x10-60.088|0.1600.5415.68x10-60.787

A question based on quantitative analysis, which is to be accomplished.

Answered: The extinction coefficient for FD&C…

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter15: Molecular Luminescence Spectrometry

Section: Chapter Questions

Problem 15.16QAP

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Blue Blue dye stock solution Absorbance at 630 nm Calibration curve Part 1: 0.1944 M 0.00697 y = 0.0682x A purple solution is prepared by mixing 5.00 mL of the blue dye solution with 6.75 mL of red dye solution. The calibration curve for each color is provided. Part 2 out of 3 (a) Determine the theoretical molar concentration of each dye in the mixture. [Blueltheoretical = 8.27 x 10-2 [Red] theoretical = 1.16 [Bluelexperimental [Redlexperimental " *10-1 M M x 10 (b) Using the absorbance and calibration curve for each dye, calculate the experimental molar concentration of each dye in the mixture. x 10 Red Red dye stock solution Absorbance at 500 nm Calibration curve 0.2015 M 0.00193 y = 0.0170x M M
Compund AFK has a maximum absorbance of 650 nm. Its molar absorptivity at 650 is 8400 M−1cm−1 and the path length is 1 cm. Using a spectrophotometer, determine the concentration of Compound AFK it is absorbance at 650 nm is 0.700 ? 8.33x10-5 mol/L 1.20 x104 mol/L 1.08 x10-3 mol/L 7.74 x10-2 mol/L
A blue product absorbs light at 590 nm (ε590nm = 7,600 M-1 cm-1). Its concentration was too concentrated to determine an accurate absorbance reading. Therefore, the solution was diluted in the following manner before reading it's absorbance. 0.500 mL was added to 1.000 mL of water to make "Dilution 1". Then 0.700 mL of "Dilution 1" was added to 3.000 mL of water to make "Dilution 2". The absorbance reading of "Dilution 2" was determined to be 0.220 when using a 1 cm pathlength. Determine the millimolar (mM) concentration of the blue product in the enzyme reaction. Indicate the concentration to the precision of 3 decimal places. ANS: 0.078 nM. Would like some steps on how to get this answer. Thanks.
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Unrounded Rounded ε∗,L/μmolε∗,L/μmol 0.0259825 0.0260 heres data: TZ # Concentration Absorbance at 430 nmnm 1 33.6480 0.931 2 25.2360 0.757 3 16.8420 0.210 4 8.41200 0.137 5 4.20600 0.122
Three compounds (A, B and C) are mixed together ( they are present in the same container). Molar absorptivity coefficients of these compounds at 575nm -1 -1 are: 2500, 3750 and 5250Lcm mol, respectively. Concentrations are: 2 × 10M for A, 5× 105 M for B and -6 1.5×10 M for C. What is the absorbance measured at 575mm, in 1.0cm cuvette of the solution containing all three compounds? Show your calculations.
The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.565. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Concentration of Blue Dye (M) 6.35x10-7 1.23x10-6 3.84x10-6 5.68x10-6 Absorbance @ 630 nm 0.088 0.160 0.541 0.787
In determining the concentration of a sample X, it was found that it has maximum absorbance at 275 nm. Compound X, has molar absorptivity at 275 nm of 8400 L / mol.cm. Using a spectrophotometer with a 1 cm cuvette, an absorbance of 0.70 was found. What is the concentration of compound X in the sample?
The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. The student also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.205. Use your Vernier Graphical Analysis to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. (Report your final answer to 3 sig figs) Concentration of Blue Dye (M) Absorbance @ 630 nm 6.35x10-7 1.23x10-6 3.84x10-6 5.68x10-6 0.088 0.160 0.541 0.787
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Specific absorbance is the absorbance of a 1% solution of a given substance. The specific absorbance can be calculated by: A 1% ст C(i % , For the identification of a substance, 0.0482 g of substance has been weighed, which has been dissolved in 50.00 ml of demineralized water. Thereafter, 50.0 µl of this solution has been diluted to 50.00 ml. a UV-wise analysis (A = 249 nm) of this dilution gave an absorbance of 0.925. a) Calculate the concentration of the diluted solution in% (w/v). b) Calculate the specific absorbance of the substance. c) Will it be possible to measure the specific absorbance directly on a 1% solution? Why / why not?

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