Specific absorbance is the absorbance of a 1% solution of a given substance. The specific absorbance can be calculated by: A A cm 1% 1 ст c(1% For the identification of a substance, 0.0482 g of substance has been weighed, which has been dissolved in 50.00 ml of demineralized water. Thereafter, 50.0 µl of this solution has been diluted to 50.00 ml. a UV-wise analysis (A = 249 nm) of this dilution gave an absorbance of 0.925. %3D a) Calculate the concentration of the diluted solution in% (w/v). b) Calculate the specific absorbance of the substance. c) Will it be possible to measure the specific absorbance directly on a 1% solution? Why /why not?

Specific absorbance is the absorbance of a 1% solution of a given substance. The specific absorbance can be calculated by: A A cm 1% 1 ст c(1% For the identification of a substance, 0.0482 g of substance has been weighed, which has been dissolved in 50.00 ml of demineralized water. Thereafter, 50.0 µl of this solution has been diluted to 50.00 ml. a UV-wise analysis (A = 249 nm) of this dilution gave an absorbance of 0.925. %3D a) Calculate the concentration of the diluted solution in% (w/v). b) Calculate the specific absorbance of the substance. c) Will it be possible to measure the specific absorbance directly on a 1% solution? Why /why not?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Concept explainers

Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

Specific absorbance is the absorbance of a 1% solution of a given substance. The specific
absorbance can be calculated by:
A
1%
ст
C(i %
,
For the identification of a substance, 0.0482 g of substance has been weighed, which has been
dissolved in 50.00 ml of demineralized water. Thereafter, 50.0 µl of this solution has been
diluted to 50.00 ml. a UV-wise analysis (A = 249 nm) of this dilution gave an absorbance of 0.925.
a) Calculate the concentration of the diluted solution in% (w/v).
b) Calculate the specific absorbance of the substance.
c) Will it be possible to measure the specific absorbance directly on a 1% solution? Why / why not?

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