Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) Egg whites contain about 88.5% water and about 10% proteins, from which ovalbumin is the major component. The eggs were cracked open, and the egg whites were separated carefully from the yolk. Stirring of the egg whites was done to break up the membrane. This was followed by filtration through cheesecloth, where a clear filtrate was obtained. About 40.0 mL of the filtered undiluted egg white was measured, and 0.10 mL of 1 M acetic acid was slowly added for every 1.0 mL of egg white with constant stirring. A turbid mixture with few amounts of white precipitate may be expected to form. These may be removed by filtering the mixture using a cheesecloth.   What is the significance of adding 1 M acetic acid to the filtered egg white?

Please written by computer source 1. We want two T-75 flasks, each with 200,000 cells in the flask that holds 20 mL of culture medium per flask. Hemocytometer counting of cells in a 1 mm sq2 area gave an average of 12 cells from a 1:9 dilution of the cell suspension.   a.) Determine the cells/mL. b.) What is the volume of your cell suspension needed to make the flasks? c.) How you would now make the flasks as a new subculture of cells for incubation?

topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) Aside from ovalbumin, what are other possible proteins which can be isolated from egg white? Give their biological significance.

please help and explain

9 and 10 please

Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria.  One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution.  Given that each bacterial…

Topic:  Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) The computed amount of powdered ammonium sulfate was added to the egg white sample portion byportion with constant stirring while submerged in an ice bath. The solution is expected to become moreturbid, and a white precipitate is expected to form. The resulting mixture was then filtered using a cheesecloth. The residue was discarded, and 30.0 mL of thefiltrate was brought from 40% to 60% saturation by adding the required amount of powdered ammoniumsulfate in the same manner as the previous addition.  After adding ammonium sulfate, the mixture was allowed to stand with occasional stirring for 30 minutes inan ice bath. The formation of a white precipitate is expected to happen QUESTION: Explain the significance of allowing the mixture to be submerged in an ice bath for a given time.

Clinical application: A 44-year-old man with HIV is receiving antibiotics through a intravenous catheter. The antibiotics are to help treat a kidney infection. The patient develops a fever. Subsequent cultures from the patient's blood, the needle tip, and from the insertion site all show growth of an organism with large oval-shaped cells. The cells reproduce by budding. (a) What is your guess about the identity of the pathogen? (b) How do you think the antibiotics may have contributed to this outcome? (c) What do you think the portal of entry was for this pathogen?

PROTEINS 1. What will happen to free arginine after being subjected to biuret analysis (is it positive or negative, include color)2. What will happen to beef extract after being subjected to ninhydrin analysis (is it positive or negative, include color)

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