You plan to stain cells with CellLight Talin-RFP in 24 hours. Now you prepare the plate and are seeding fibroblasts HDFS 04 in 96-well plate. Seeding density is 1800 cells/well, HDFS 04 will double every 18 hours. Calculate the appropriate volume of CellLight to be added to each well, if you know that there are 150 million baculoviral vectors in one mL of CellLight, and you need to achieve 45 viral vectors per fibroblast O a. 0.126 µl O b. 0.0126 ml O. 0.0054 mL O d. 0.54 µl O e. 1.26 µl O f. 0.054 mL
You plan to stain cells with CellLight Talin-RFP in 24 hours. Now you prepare the plate and are seeding fibroblasts HDFS 04 in 96-well plate. Seeding density is 1800 cells/well, HDFS 04 will double every 18 hours. Calculate the appropriate volume of CellLight to be added to each well, if you know that there are 150 million baculoviral vectors in one mL of CellLight, and you need to achieve 45 viral vectors per fibroblast O a. 0.126 µl O b. 0.0126 ml O. 0.0054 mL O d. 0.54 µl O e. 1.26 µl O f. 0.054 mL
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:You plan to stain cells with CellLight Talin-RFP in 24 hours. Now you prepare the plate and are seeding fibroblasts HDFS 04 in 96-well
plate. Seeding density is 1800 cells/well, HDFS 04 will double every 18 hours. Calculate the appropriate volume of CellLight to be added
to each well, if you know that there are 150 million baculoviral vectors in one mL of CellLight, and you need to achieve 45 viral vectors
per fibroblast
O a. 0.126 µl
O b. 0.0126 mL
0.0054 ml
O d. 0.54 µL
1.26 µl
Of.
0.054 mL
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