A team of R&D scientists were trying to make a "better" enzyme. A molecular biologist mutated ami residues of an existing enzyme. An enzymologist on the team evaluated the engineered enzymes by m the dissociation constants between a substrate-analog and the different engineered enzymes. The engir measured Vmax of each enzyme, using a natural substrate of the enzyme. Which engineered enzyme is and why? Provide a thermodynamic explanation. Include a reaction-progress energy diagram in your Engineered Enzyme ID KA (uM) Vmax (uM min!) A 10 150
A team of R&D scientists were trying to make a "better" enzyme. A molecular biologist mutated ami residues of an existing enzyme. An enzymologist on the team evaluated the engineered enzymes by m the dissociation constants between a substrate-analog and the different engineered enzymes. The engir measured Vmax of each enzyme, using a natural substrate of the enzyme. Which engineered enzyme is and why? Provide a thermodynamic explanation. Include a reaction-progress energy diagram in your Engineered Enzyme ID KA (uM) Vmax (uM min!) A 10 150
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Transcribed Image Text:A team of R&D scientists were trying to make a "better" enzyme. A molecular biologist mutated amino acid
residues of an existing enzyme. An enzymologist on the team evaluated the engineered enzymes by measuring
the dissociation constants between a substrate-analog and the different engineered enzymes. The engineer also
measured Vmax of each enzyme, using a natural substrate of the enzyme. Which engineered enzyme is a failure
and why? Provide a thermodynamic explanation. Include a reaction-progress energy diagram in your answer.
Engineered Enzyme ID
Ka (uM)
Vmax (uM min!)
10
150
0.4
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