9. The NUDT18 protein was purified by affinity chromatography (Hiss tagged) followed by gel filtration. Explain how these steps will isolate the NUDT18 protein. 10. After purification, the NUDT18 protein was analyzed by SDS-PAGE (image shown to the right). Lane 4 contains the NUDT18 protein. a. Evaluate whether the purification was effective or not. How do you know? b. What is the size (in kD) of the NUDT18 protein? How do you know? kDa 75 50 37 25 20 15 10 1234

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