Through acid hydrolysis for 24 hours, Jackson obtained the following amino aci residues: Ala-Arg-Cys-Gln-Lys-Phe-Tyr-Val No phenylthiohydantoin structure was obtained after Edman analysis. Furthermore, no C-terminal residue was also obtained after treatment with carboxypeptidase. He then used trypsin and chymotrypsin enzymes as cleaving agents. Trypsin cleaves at the C-terminal of basic amino acids Lys and Arg while chymotrypsin cleaves at the C-terminal of aromatic amino acids such as Phe, Tyr, and Trp. The results of trypsin and chymotrypsin cleavage were listed in the table below: Enzyme Amino Acid Residues Chymotrypsin Trypsin Determine the sequence of the polypeptide using the one-letter code of the amin acids. Kindly check the lecture slide for the amino acid one-letter codes. Drag th correct boxes to their respective markers. The directionality of the N-to-C terminals is also specified below. N-terminal Val-Lys-Ala-Phe + Gln-Arg-Cys-Tyr Cys-Tyr-Val-Lys + Ala-Phe-Gln-Arg I KA|SR|YP|LF|C|T V C-terminal 1

Through acid hydrolysis for 24 hours, Jackson obtained the following amino aci residues: Ala-Arg-Cys-Gln-Lys-Phe-Tyr-Val No phenylthiohydantoin structure was obtained after Edman analysis. Furthermore, no C-terminal residue was also obtained after treatment with carboxypeptidase. He then used trypsin and chymotrypsin enzymes as cleaving agents. Trypsin cleaves at the C-terminal of basic amino acids Lys and Arg while chymotrypsin cleaves at the C-terminal of aromatic amino acids such as Phe, Tyr, and Trp. The results of trypsin and chymotrypsin cleavage were listed in the table below: Enzyme Amino Acid Residues Chymotrypsin Trypsin Determine the sequence of the polypeptide using the one-letter code of the amin acids. Kindly check the lecture slide for the amino acid one-letter codes. Drag th correct boxes to their respective markers. The directionality of the N-to-C terminals is also specified below. N-terminal Val-Lys-Ala-Phe + Gln-Arg-Cys-Tyr Cys-Tyr-Val-Lys + Ala-Phe-Gln-Arg I KA|SR|YP|LF|C|T V C-terminal 1

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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