Through acid hydrolysis for 24 hours, Jackson obtained the following amino aci residues: Ala-Arg-Cys-Gln-Lys-Phe-Tyr-Val No phenylthiohydantoin structure was obtained after Edman analysis. Furthermore, no C-terminal residue was also obtained after treatment with carboxypeptidase. He then used trypsin and chymotrypsin enzymes as cleaving agents. Trypsin cleaves at the C-terminal of basic amino acids Lys and Arg while chymotrypsin cleaves at the C-terminal of aromatic amino acids such as Phe, Tyr, and Trp. The results of trypsin and chymotrypsin cleavage were listed in the table below: Enzyme Amino Acid Residues Chymotrypsin Trypsin Determine the sequence of the polypeptide using the one-letter code of the amin acids. Kindly check the lecture slide for the amino acid one-letter codes. Drag th correct boxes to their respective markers. The directionality of the N-to-C terminals is also specified below. N-terminal Val-Lys-Ala-Phe + Gln-Arg-Cys-Tyr Cys-Tyr-Val-Lys + Ala-Phe-Gln-Arg I KA|SR|YP|LF|C|T V C-terminal 1

Through acid hydrolysis for 24 hours, Jackson obtained the following amino aci residues: Ala-Arg-Cys-Gln-Lys-Phe-Tyr-Val No phenylthiohydantoin structure was obtained after Edman analysis. Furthermore, no C-terminal residue was also obtained after treatment with carboxypeptidase. He then used trypsin and chymotrypsin enzymes as cleaving agents. Trypsin cleaves at the C-terminal of basic amino acids Lys and Arg while chymotrypsin cleaves at the C-terminal of aromatic amino acids such as Phe, Tyr, and Trp. The results of trypsin and chymotrypsin cleavage were listed in the table below: Enzyme Amino Acid Residues Chymotrypsin Trypsin Determine the sequence of the polypeptide using the one-letter code of the amin acids. Kindly check the lecture slide for the amino acid one-letter codes. Drag th correct boxes to their respective markers. The directionality of the N-to-C terminals is also specified below. N-terminal Val-Lys-Ala-Phe + Gln-Arg-Cys-Tyr Cys-Tyr-Val-Lys + Ala-Phe-Gln-Arg I KA|SR|YP|LF|C|T V C-terminal 1

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

Carboxypeptidase requires a Zn²+ cofactor for the hydrolysis of the peptide bond of a C-terminal aromatic amino acid. T/F?
MCQ 21: After the substrate diffuses with the enzyme, the next action in series is the A. amino acids may acts as nucleophiles B. products diffuse away from the active site C. enzyme-substrate complex is binded D. product dissolves in capillaries and is carried away.
The term used to separate a mixture of enantiomers of amino acids using chiral reagents? A) Thermodynamic resolution B) Meso resolution C) Kinetic Resolution D) Neutralization E) Isomeric resolution
20. After treatment with peroxyformic acid, the peptide hormone vasopressin is partially hydrolyzed. The following fragments are recovered. Propose a structure for vasopressin. Phe-Gln-Asn Pro-Arg-Gly NH, Tyr-Phe-Gln-Asn Cys-Tyr-Phe Asn-Cys-Pro-Arg
Brucine is a poisonous alkaloid obtained from Strychnos nux vomica, a tree that grows in India, Sri Lanka, and northern Australia. Write out a resolution scheme similar to the one given in Section 29.3A, which shows how a racemic mixture of phenylalanine can be resolved using brucine.
Draw the structure of the N-phenylthiohydantoin formed by initial Edman degradation of each peptide: (a) Ala–Gly–Phe–Phe; (b) Val–Ile–Tyr.
For the following peptide there are [ Select] acids present and [ Select] + H3N carbons present? Make sure to check the sidechains for chiral carbons as well. SH amino ◆ chiral H N 00 OO
Glutamic acid has three protonatable groups (two carboxyls and one amino). Calculate its isoelectric point, indicating what values of pKa you used to calculate it.
Given the fact that phenylalanine has a pK₁1-1.83 and pK₂2-9.13, show the condensed structure of the dominant specie in solution at a. Determine the isoelectric point for valine. Show your work. b. pH=1.0 c. pH=11.0
Phosphoric acid has pka values of approximately 2, 7 and 12. What are the relevant pka for the phosphodiester contained in sn-glycero-3-phosphocholine, a natural glycerophospholipid? O 2 only O 2,7, and 12 O None are correct O2, and 7 only O7 and 12 only