A sample of an unknown peptide was divided into two aliquots. One aliquot was treated with trypsin; the other was treated with cyanogen bromide. Given the following sequences (N-terminal to C- terminal) of the resulting fragments, deduce the sequence of the original peptide. Trypsin treatment Asn-Thr-Trp-Met-lle-Lys Gly-Tyr-Met-Gln-Phe Val-Leu-Gly-Met-Ser-Arg Cyanogen bromide treatment Gln-Phe Val-Leu-Gly-Met lle-Lys-Gly-Tyr-Met Ser-Arg-Asn-Thr-Trp-Met

Chemistry
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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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A sample of an unknown peptide was divided into
two aliquots. One aliquot was treated with trypsin;
the other was treated with cyanogen bromide.
Given the following sequences (N-terminal to C-
terminal) of the resulting fragments, deduce the
sequence of the original peptide.
Trypsin treatment
Asn-Thr-Trp-Met-lle-Lys
Gly-Tyr-Met-Gln-Phe
Val-Leu-Gly-Met-Ser-Arg
Cyanogen bromide treatment
Gin-Phe
Val-Leu-Gly-Met
lle-Lys-Gly-Tyr-Met
Ser-Arg-Asn-Thr-Trp-Met
Transcribed Image Text:A sample of an unknown peptide was divided into two aliquots. One aliquot was treated with trypsin; the other was treated with cyanogen bromide. Given the following sequences (N-terminal to C- terminal) of the resulting fragments, deduce the sequence of the original peptide. Trypsin treatment Asn-Thr-Trp-Met-lle-Lys Gly-Tyr-Met-Gln-Phe Val-Leu-Gly-Met-Ser-Arg Cyanogen bromide treatment Gin-Phe Val-Leu-Gly-Met lle-Lys-Gly-Tyr-Met Ser-Arg-Asn-Thr-Trp-Met
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