Three different Hfr strains, derived from the same F* strains (H), were mated with F strains auxotrophic for a number of nutritional requirements (trp bio leu azi mal tyr gal ). Matings were interrupted at various intervals and cells were plated on minimal medium supplemented with particular nutrients to test for gene transfer. The following mapping data was obtained: Genes azi * mal Hfr strains trp 17 bio leu gal* tyr Hfr H Hfr 1 Hfr 2 Hfr 3 Hfr 4 25 4 10 10 18 3 16 14 22 11 15 19 19 4 8 Draw a circular map of E. coli chromosome. Include distances (in minutes) between genes in your map and label arrowheads to show the origin of transfer for the five Hfr strains.
Three different Hfr strains, derived from the same F* strains (H), were mated with F strains auxotrophic for a number of nutritional requirements (trp bio leu azi mal tyr gal ). Matings were interrupted at various intervals and cells were plated on minimal medium supplemented with particular nutrients to test for gene transfer. The following mapping data was obtained: Genes azi * mal Hfr strains trp 17 bio leu gal* tyr Hfr H Hfr 1 Hfr 2 Hfr 3 Hfr 4 25 4 10 10 18 3 16 14 22 11 15 19 19 4 8 Draw a circular map of E. coli chromosome. Include distances (in minutes) between genes in your map and label arrowheads to show the origin of transfer for the five Hfr strains.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
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Transcribed Image Text:Three different Hfr strains, derived from the same F strains (H), were mated with F- strains
auxotrophic for a number of nutritional requirements (trp bio leu azi mal tyr gal ).
Matings were interrupted at various intervals and cells were plated on minimal medium
supplemented with particular nutrients to test for gene transfer. The following mapping data
was obtained:
Genes
Hfr strains
bio
leu
azi
mal
gal
trp
17
tyr
Hfr H
Hfr 1
Hfr 2
Hfr 3
Hfr 4
25
4
10
10
18
3
5
9.
1
16
14
22
15
3
19
19
11
4
8
Draw a circular map of E. coli chromosome. Include distances (in minutes) between genes in
your map and label arrowheads to show the origin of transfer for the five Hfr strains.
(10
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