The methodology for enumerating coliforms using the most probable number method calls for presumptive and confirmatory steps. Discuss the reasons why one cannot go directly to inoculation of brilliant green lactose bile broth after homogenizing the sample.

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.