The map shows a wild-type HOAP gene, whereas Mutant 1 has a DNA deletion from base pair 1125 to 1445 and Mutant 2 contains a point mutation at 1372. Predict the sizes of DNA fragments that would be generated in each of the following digests. Wild-type (BamHI): Wild-type (EcoRI): Wild-type (BamHI + EcoRI): Mutant 1 (BamHI): Mutant 1 (EcoRI): Mutant 1 (BamHI + ECORI): Mutant 2 (BamHI): Mutant 2 (EcoRI): Mutant 2 (BamHI + EcoRI): BamHI O EcoRI 740 Promoter ECORI 1140 HOẠP HOAP HP1/ORC Associated Protein 7200 bp Saci 2100 BamHI 2420 Psti 2700 Hindll 3440

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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The map shows a wild-type HOAP gene, whereas Mutant 1 has a DNA deletion from base pair 1125 to 1445 and Mutant 2 contains a point mutation at 1372. Predict the sizes of DNA fragments that would be generated
in each of the following digests.
Wild-type (BamHI):
Wild-type (EcoRI):
Wild-type (BamHI + EcoRI):
Mutant 1 (BamHI):
Mutant 1 (EcoRI):
Mutant 1 (BamHI + EcoRI):
Mutant 2 (BamHI):
Mutant 2 (EcoRI):
Mutant 2 (BamHI + EcoRI):
BamHI O
EcoRI 740
Promoter
ECORI 1140
HOAP
HOAP
HP1/ORC Associated Protein
7200 bp
Sacl 2100
BamHI 2420
Psti 2700
Hindlll 3440
Transcribed Image Text:The map shows a wild-type HOAP gene, whereas Mutant 1 has a DNA deletion from base pair 1125 to 1445 and Mutant 2 contains a point mutation at 1372. Predict the sizes of DNA fragments that would be generated in each of the following digests. Wild-type (BamHI): Wild-type (EcoRI): Wild-type (BamHI + EcoRI): Mutant 1 (BamHI): Mutant 1 (EcoRI): Mutant 1 (BamHI + EcoRI): Mutant 2 (BamHI): Mutant 2 (EcoRI): Mutant 2 (BamHI + EcoRI): BamHI O EcoRI 740 Promoter ECORI 1140 HOAP HOAP HP1/ORC Associated Protein 7200 bp Sacl 2100 BamHI 2420 Psti 2700 Hindlll 3440
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