The isoelectric point of eIF4a is 5.02. Students are given a sample of cell lysate containing eIF4A at pH 7.4. They are also provided with two buffers of pH 7.4 to use for ion exchange chromatography. One buffer has a very high salt concentration (1 M), the other has a low salt concentration (0.1 M). Mariela decides to use an anion exchange column, while Ashok chooses a cation exchange column. Who made the better decision? Provide a detailed explanation of why one student will end up with a purer sample of eIF4A.    Which buffer will you use while washing away impurities (high or low salt), and which would you use to remove eIF4A from the column? Explain your choice.

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Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.