The following values were taken from the Richard's table on the thermal death kinetics of B. stearothermophi. (A=4.9x1037 min¹, E=67.48 kcal/mol, R=1.987 cal/mol K): Temperature, °C 120 121 125 130 Specific death rate The cumulative del factor constant k, min-¹ V 1.47 1.83 4.35 12.55 7.25 9.08 22.08 65.10 (i) The time taken for heating, cooling and holding periods in the sterilisation of a batch fermentor medium for the destruction of B. stearothermophilus were: time to heat from 100 to 121°C = 30 min time to cool from 121 to 100 °C = 16 min time of holding at 121 °C = 12 min Calculate the destruction ratio (No/N) for the process. (ii) If the sterilisation were to be carried out continuously at 130 °C, and the heating and cooling characteristics were: time to heat from 100 to 130 °C = 2 min time to cool from 130 to 100 °C = 1 min what holding time would be required to achieve the same ratio of (No/N) as in section (1) above? State all the assumptions made in your calculations.

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The following values were taken from the Richard's table on the thermal death
kinetics of B. stearothermophi.
cal/mol K):
(A=4.9x1037min', E=67.48 kcal/mol, R=1.987
Temperature, °C
rate The cumulative del factor
Specific
constant k, min
death
-1
V
120
1.47
7.25
121
1.83
9.08
125
4.35
22.08
130
12.55
65.10
(i) The time taken for heating, cooling and holding periods in the sterilisation of a
batch fermentor medium for the destruction of B. stearothermophilus were:
time to heat from 100 to 121°C = 30 min
time to cool from 121 to 100 °C =16 min
time of holding at 121 °C
= 12 min
Calculate the destruction ratio (No/N) for the process.
(ii) If the sterilisation were to be carried out continuously at 130 °C, and the
heating and cooling characteristics were:
time to heat from 100 to 130 °C = 2 min
time to cool from 130 to 100 °C = 1 min
what holding time would be required to achieve the same ratio of (No/N) as in
section (i) above? State all the assumptions made in your calculations.
Transcribed Image Text:The following values were taken from the Richard's table on the thermal death kinetics of B. stearothermophi. cal/mol K): (A=4.9x1037min', E=67.48 kcal/mol, R=1.987 Temperature, °C rate The cumulative del factor Specific constant k, min death -1 V 120 1.47 7.25 121 1.83 9.08 125 4.35 22.08 130 12.55 65.10 (i) The time taken for heating, cooling and holding periods in the sterilisation of a batch fermentor medium for the destruction of B. stearothermophilus were: time to heat from 100 to 121°C = 30 min time to cool from 121 to 100 °C =16 min time of holding at 121 °C = 12 min Calculate the destruction ratio (No/N) for the process. (ii) If the sterilisation were to be carried out continuously at 130 °C, and the heating and cooling characteristics were: time to heat from 100 to 130 °C = 2 min time to cool from 130 to 100 °C = 1 min what holding time would be required to achieve the same ratio of (No/N) as in section (i) above? State all the assumptions made in your calculations.
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