The following diagram shows one-half of a restriction site.(a) Draw the other half.GAC G I C(b) Use heavy arrows (↑1) to identify type II cleavage sites thatwould yield blunt-ended duplex DNA products.(c) Use light arrows (T1) to identify type II cleavage sites yieldingstaggered cuts that could be converted directly to recombinant DNAmolecules by DNA ligase, with no other enzymes involved.(d) If this were the recognition site for a type I restriction endonu-clease, where would cutting of the duplex occur?(e) If DNA sequences were completely random, how large an inter-val (in kilobase pairs) would you expect between identical copies ofthis sequence in DNA?

Introduction: The genetic material in the living organism that transfer from one generation to…

Answered: The following diagram shows one-half of…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

To promote genetic diversity in bacteria, it has been found that some species have a genetic mechanism that allows them to increase their mutation rate during DNA replication. A scientist hypothesizes that the functions of the following two enzymes would be impacted by this mechanism. In each case, state if you agree and provide a reason for your answer. i) DNA primaseii) DNA polymerase III
A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid map is depicted in the diagram below and the Sau3A (S) restriction sites are indicated. Which of the following DNA fragments do you expect to see on an agarose gel when you run Sau3A-digested plasmid ‘BS1030’ DNA? a. 250 bp, 450 bp, 550 bp, 1.1 kb, 1.5 kb and 2.0 kb b. 2.0kb c. 250 bp, 400 bp, 450 bp, 500 bp and 550 bp d. 100 bp, 200 bp, 250 bp, 400 bp, 500 bp and 550 bp
Please label the following: (A) INITIATION *Complementary strand *Template Strand *RNA polymerase *Initiation Site *Termination Site *Promoter *5' and 3' end on each DNA strand Initiation (A) FRA FORÆTSIST Elongation (B) (C) restorag Termination IND PLATIN RNA (B) ELONGATION *Exiting DNA *Exiting RNA Transcript *Template Strand *Nucleotides (ATP, UTP, CTP, GTP) *Direction of transcription PHILLI 13' DIDI
You isolate a mouse Tau-gene-containing DNA fragment from the chicken and hybridize it to the freshly-made and isolated hnRNA (primary transcript) from the nucleus of the mouse cells transcribed from the Tau gene (immediately after it was produced), allowing no time for processing of the hnRNA. Describe what you see when you look at the DNA/RNA hybrid molecule under the electron microscope.
You are trying to clone a gene. You have successfully isolated it from the genomic DNA of an organism using the Hindlll restriction enzyme. You then take a plasmid with a single EcoRI restriction site and cleave it with EcoRI. You combine these two fragments and treat them with DNA ligase. Answer the two questions below. a.(2 points Does the cloning reaction succeed as described? If so, what is the product obtained? b. Explain your answer above.
3b) Briefly explain what telomerase does, how it accomplishes what it does, and why that allows a cell to completely and accurately replicate the ends of linear DNA molecules. (please note that the question does not ask you to explain the entire process of replication of the end of a linear DNA strand, it only asks about the function of telomerase in this process)
a.) wanting to clone gene Z into pVector, the gene is amplified by PCR and restriction sites are added to the flanking ends. without realizing that the antibotic resistant gen )tetR) had a Sal1 site, you decide to add EcoR1 and Sal1 recofnition sequencen into the 12-nucleotide primers. Write the sequence of the 2 primers, noting the 5' and 3' ends?
Many of the gene products involved in DNA synthesis wereinitially defined by studying mutant E. coli strains that could notsynthesize DNA. Question: The dnaE gene encodes the a subunit of DNA polymeraseIII. What effect is expected from a mutation in this gene?How could the mutant strain be maintained?
Ligation is an essential step in the cloning process. It refers to the joining of the gene of interest to the vector using DNA ligase. (i) Determine FOUR (4) control groups which are important to determine the success of this step and also to troubleshoot if any problem should occur. In an experiment, 100 ng vector was added into ligation reaction with 50 ng of insert (500 bp). The desired vector: insert ratio of 1: 3 was used. Determine the size of the (ii) vector.
I have complete the table that required. Indicate which mutations were success why some were not from the size about. indicate at what stage any of the mutagenesis failed.

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