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The figure below represents two DNA molecules, with polarities as indicated. The dashed block shows the region of DNA which you wish to amplify by PCR. In each case, indicate the positions of the forward and reverse primers you would use for the PCR, their polarities, and the direction of synthesis of the new strands with an arrow at the end of the primer. 5' 3' ------------------- 3' 3' 5' 5' ------------------------------ 5' 3'

The figure below represents two DNA molecules, with polarities as indicated. The dashed block shows the region of DNA which you wish to amplify by PCR. In each case, indicate the positions of the forward and reverse primers you would use for the PCR, their polarities, and the direction of synthesis of the new strands with an arrow at the end of the primer. 5' 3' ------------------- 3' 3' 5' 5' ------------------------------ 5' 3'

Concepts of Biology
Concepts of Biology
1st Edition
ISBN:9781938168116
Author:Samantha Fowler, Rebecca Roush, James Wise
Publisher:Samantha Fowler, Rebecca Roush, James Wise
Chapter9: Molecular Biology
Section: Chapter Questions
Problem 15CTQ: Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’
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The figure below represents two DNA molecules, with polarities as indicated. The dashed block shows the region of DNA which you wish to amplify by PCR. In each case, indicate the positions of the forward and reverse primers you would use for the PCR, their polarities, and the direction of synthesis of the new strands with an arrow at the end of the primer. 5' 3' ------------------- 3' 3' 5' 5' ------------------------------ 5' 3'
Transcribed Image Text:The figure below represents two DNA molecules, with polarities as indicated. The dashed block shows the region of DNA which you wish to amplify by PCR. In each case, indicate the positions of the forward and reverse primers you would use for the PCR, their polarities, and the direction of synthesis of the new strands with an arrow at the end of the primer. 5' 3' ------------------- 3' 3' 5' 5' ------------------------------ 5' 3'
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