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The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP experiment with purified liposomes. The FRAP recovery curves are shown below. Fluorescence intensity RO Time Pre-bleaching Bleaching imaging imaging Time Post-bleaching imaging с Time D Time (a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D? (b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.

The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP experiment with purified liposomes. The FRAP recovery curves are shown below. Fluorescence intensity RO Time Pre-bleaching Bleaching imaging imaging Time Post-bleaching imaging с Time D Time (a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D? (b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Example Problem: FRAP Data Interpretation The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP experiment with purified liposomes. The FRAP recovery curves are shown below. Fluorescence intensity ROI A 3 Time B Time Post-bleaching imaging с Time D Time Pre-bleaching Bleaching imaging imaging (a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D? (b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.
Transcribed Image Text:Example Problem: FRAP Data Interpretation The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP experiment with purified liposomes. The FRAP recovery curves are shown below. Fluorescence intensity ROI A 3 Time B Time Post-bleaching imaging с Time D Time Pre-bleaching Bleaching imaging imaging (a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D? (b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.
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