4) Two membrane preparations have been made from the plasma membrane of erythrocytes (i.e red blood cells)and are being maintained in an isotonic buffer solution: (1) right-side-out membrane vesicles, in which theexoplasmic leaflet of the vesicle faces the buffer (equivalent in orientation to that of the exoplasmic leaflet of theintact plasma membrane); and (2) inside-out membrane vesicles in which the exoplasmic leaflet faces the interiorof the vesicles and the cytoplasmic leaflet faces the buffer.80a.Right-side-out vesicles(ROVS)Very Low FluorescenceInside-out vesicles(IOVS)Very High FluorescenceA newly discovered phosphoglyceride, called phosphotidylalanine is currently being studied. AnnexinV is a protein that binds to phosphatidylalanine, which is one of the phospholipids present in the cellmembrane preparations described above. Fluorescently labeled Annexin V is mixed with each vesiclepreparation. The vesicles are washed to remove unbound Annexin V and then examined for levels offluorescence. Presence of Annexin V is detected by the level of fluorescence, as shown above. What can bededuced from these data?

The data describes an experiment on two vesicle preparations derived from erythrocyte plasma…

Answered: 4) Two membrane preparations have been…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

Explain how salt concentrations contribute to the cell membrane intergrity in cells? What are the three different scenarios that could occur and why?
For a lab on diffusion and osmosis, where a egg yolk was placed in a cup water the following question asks Consider a scenario in which the size of an egg yolk remains unchanged after in water soaking for an hour. What are two possible explanations as to why this occurs? What would two reasons be for the yolk to be unchanged after a hour in water?
Name: Period: Date: Passive Cellular Transport Assignment- Student Edition IV. The image below shows red blood cells placed in three different solutions - hypotonic, hypertonic and isotonic. a) Label each sample of red bicod cells with the terms hypotonic, hypertonic and isotonic to show the type of solution they have been placed in. b) Explain the effect of each type of sclution on the red blood cells: Hypotonic: Hypertonic. Isotonic The same treatment was applied to three plant cells (shown in the diagrams below). H,O H,0 HO c) For each plant cell, identify the type of solution has been placed in and the effect it has on the cell. d) Identify the type of solution that is best suited for both plant and animal cells. Give a reason for your answer. iTeachly Copyright © iTeachly.com 3
Dr Renko studied diffusion of tracer molecules to study paracellular diffusion across an epithelial monolayer of Caco-2 cells. The tracers were fluorescently labelled dextran molecules of different molecular weight. She is screening various drugs to determine whether they affect the cells in any way. Which drugs are most likely to change paracellular diffusion? a.) Drugs that modify microvilli are the most likely to change paracellular diffusion b.) Drugs that modify gap junctions are the most likely to change paracellular diffusion c.) Drugs that modify with aquaporins are the most likely to change paracellular diffusion d.) Drugs that modify tight junctions are the most likely to change paracellular diffusion
A) Based on the drawings above, which molecules were able to diffuse through the 150 MWCO membrane? B) Which molecules(s) were not able to diffuse? Why not? C) Which side of the chamber had the greater osmolarity/osmotic pressure and how could you tell?
The following table depicts the rate of transport of a molecule into a cell as the external concentrations are changed. Based on what you know about the characteristics of membrane transport, the molecule is most likely transported by: a) passive transport b) simple diffusion across the membrane c) facilitated diffusion d) active transport
The three fundamental classes of proteins involved in transport across membranes are: i) channelsii) pumpsiii) exchangers For each class, describe its essential features, explain how transport is accomplished and name at least one representative example.
Statement 1: The direction of diffusion of a solute across a membrane is determined by its concentration gradient and always moves toward equilibrium. Statement 2: In passive transport, solutes move down the gradient from a region of low concentration to a region of high concentration. A Both statements are true. Both statements are false. C) Statement 1 is true. Statement 2 is false. Statement 1 is false. Statement 2 is true.
Which of the following best describes the pH of the lysosomal interior? a.) The interior of a lysosome generally has a pH similar to cytoplasm b.) The interior of a lysosome generally has a pH higher than cytoplasm c.) The interior of a lysosome generally has a pH lower than cytoplasm
The transport of a molecule is investigated using two chambers (left and right) separated by a synthetic membrane containing transport proteins. A solution containing varying concentrations of the molecule is added to the left side while pure water is added to the right. The transport rate of the molecule is determined by measuring the concentration of molecule that accumulates on the right side. The following table summarizes the transport rate of the molecule at various concentrations. Based on this you can conclude: a) the molecule is most likely transported by facilitated diffusion b) the molecule is moving across the membrane by passive transport c) the membrane is freely permeable to the molecule d) the molecule is most likely transported by active transport
QUESTION 1 a) Figure 2 shows the fluid mosaic model of plasma membrane. Figure 2 i) Name the parts labelled A and B. A: .. ii) State the component and function of structure C. Component Function: b) Movement of solutes across a membrane occur with the help of transport protein. i) Name the disease due to defective carrier proteins that transport cystine. i) Name and describe one (1) type of gated ion channel. i) Explain how glucose molecules are transported across plasma membrane into a cell via passive transport.
In terms of their effect on the rate of diffusion, which of the following is the odd one out? a) decreasing the surface area of the membrane b) decreasing membrane permeability c) decreasing lipid solubility of the diffusing molecule d) decreasing the concentration gradient of the diffusing molecule e) decreasing the thickness of the membrane

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