11. Treatment of disrupted erythrocyte membranes with a concentrated salt solution released an unknown membrane protein, X. Proteolytic enzymes cleaved X into fragments. In additional experiments, intact erythrocytes were treated with proteolytic enzymes, washed, then disrupted. Extraction of membrane components yielded intact X. What do these observations indicate about the location of X in the plasma membrane? Do the properties of X resemble those of an integral membrane protein or a peripheral membrane protein?

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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11. Treatment of disrupted erythrocyte membranes with a concentrated salt solution released an unknown
membrane protein, X. Proteolytic enzymes cleaved X into fragments. In additional experiments, intact
erythrocytes were treated with proteolytic enzymes, washed, then disrupted. Extraction of membrane
components yielded intact X. What do these observations indicate about the location of X in the plasma
membrane? Do the properties of X resemble those of an integral membrane protein or a peripheral
membrane protein?
Transcribed Image Text:11. Treatment of disrupted erythrocyte membranes with a concentrated salt solution released an unknown membrane protein, X. Proteolytic enzymes cleaved X into fragments. In additional experiments, intact erythrocytes were treated with proteolytic enzymes, washed, then disrupted. Extraction of membrane components yielded intact X. What do these observations indicate about the location of X in the plasma membrane? Do the properties of X resemble those of an integral membrane protein or a peripheral membrane protein?
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