The diagram to the right represents one technique used in biotechnology. The organic compound used to cut the bacterial DNA so that the human DNA could be inserted a(n)

Q: Why do we need to use salt solution when we cloning dna?

A: Once the genomic deoxyribonucleic acid is certain to the silicon oxide membrane, the supermolecule…

Q: PCR is a very useful method in biotechnology because it does not require the 6 or more different key…

A: PCR is a polymerase chain reaction- it ia process of replication of DNA in laboratory. PCR can…

Q: Enzymes of bacterial origin used in a wide variety of techniques are: ligases restriction…

A: DNA polymerase

Q: PCR has many useful applications. However, an incorrect application of PCR is: a) Amplification of…

A: DNA or Deoxyribonucleic acid is a macromolecule which is responsible for the transfer of hereditary…

Q: Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and  70%…

A: 70% ethanol is used in washing DNA furing DNA extraction. 100% ethanol is used in the final step. In…

Q: The other options are:   a. RNA cannot be digested by restriction enzymes b. RNA is small enough…

A: Northern blotting involve separation of RNA fragments by gel electrophoresis. In contrast Southern…

Q: Match the term with its description.

A: There are various enzymes and gene components that are studied in techniques of genetic engineering.…

Q: After properly troubleshooting, you finally are able to visualize the gel. You obtained the image…

A: DNA extraction is a protocol of isolating genomic DNA from any cell type. Generally we use the…

Q: DNA scissors used in genetic engineering applications are called Endo nucleases Restriction enzymes…

A:

Q: Explain Shortly. I need help   The emergence of new molecular biology techniques has allowed…

A: You have asked three parts of a question. Third part is a personal opinion on this topic, so I won't…

Q: 2) A scientist wants to increase the amount of sample of a DNA sequence of 100 bps. Calculate the…

A: Given that molar mass of average DNA base pair is 600g/mol. That means 600g of base pairs contain…

Q: Which of the following ingredients does not belong in a PCR reaction? DNA polymerase B) Primer…

A: Polymerase chain reaction is a method used to produce millions of copies of any DNA sample and hence…

Q: A student carries out PCR using the following steps:  step 1: 94C for 1 minute Step 2: 60C for…

A: A complete set of chromosomes in any species is regarded as its genome. Each  chromosomes homes DNA…

Q: Write the 3' to 5' DNA bottom strand • Write the mRNA sequence of top strand Write the mRNA sequence…

A: In DNA   there are four nitrogen as base that is adenine, Thymine, guanine and cytosine is present.…

Q: You are cloning a gene from a eukaryotic organism into a plasmid to produce a recombinant protein in…

A: The biological process by which naked DNA (deoxyribonucleic acid) gets transferred from one…

Q: Which of the following most accurately describes the process of DNA cloning?​ set of laboratory…

A: Cloning requires DNA with desirable genes obtained using restriction enzymes which is then…

Q: Once separated from other cellular components, extracted DNA can be used for a variety of purposes.…

A: Introduction:- Biotechnology relies heavily on the extraction of DNA. It serves as the foundation…

Q: Select all the following statements that are correct regarding agarose gel electrophoresis and…

A: Agarose gel electrophoresis is a process used for separation of DNA fragments. Steps of agarose gel…

Q: Transformation can be described as:

A: Prokaryotes are unicellular organisms that reproduce in two ways namely- Asexual reproduction and…

Q: Polymerase Chain Reaction (PCR) works by exposing the reaction to a series of temperature changes.…

A: Polymerase chain reaction (PCR) is a scientific method that exponentially multiplies a single copy…

Q: 16. Which of the following enzyme repairs the DNA backbone during molecular cloning (that is you are…

A: Answer: Introduction: Molecular cloning is a genetic methods in biotechnology, which are utilized to…

Q: Please answer these two questions regarding PCR:   a) Why do you need to perform PCR on DNA obtained…

A: The polymerase chain reaction (PCR) is a technique used to make many copies of a specific segment of…

Q: Answers to the following questions. A. You are given a tube containing 275 ng of purified PCR…

A: 5) A. Amount of purified PCR product DNA in the tube = 275 ng = 275 000 pg (1000 pg = 1 ng) The…

Q: Above are the results of gel electrophoresis following digestion with restriction enzymes. What is…

A: Using specialized enzymes known as restriction endonucleases, restriction digestion is a laboratory…

Q: STR #1: D5S818 allele 1: 5'-CAATCATAGCCACAA | GATAGATAGATAGATAGATAGATAGATACCAAAGAG-3' allele 2:…

A: DNA profiling is a laboratory forensic technique used to obtain the specific pattern of DNA from a…

Q: Perform the Megaprimer PCR procedure (in scheme A). Indicates the number of Primers required in the…

A: The Megaprimer PCR method is a technique used for introducing specific mutations, deletions, or…

Q: PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein…

A: Introduction PCR is a method for amplifying a specific region of DNA from a mixed template. Reagents…

Q: MOLECULAR CLONING • Molecular cloning is the laboratory process used to create recombinant DNA. • It…

A: Recombinant DNA: it is a method of combining two distinct DNA (molecule) sequences, which may or may…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.