You want to study a biomolecule in the laboratory. You have ordered the synthetic gene from a company and want to check that it has been introduced into your model organism. What is the proper order of experiments below for doing this? I. Sanger sequencing II. Transformation III. Restriction digest IV. DNA purification from a single colony I, II, III, IV I, IV, II, III III, II, IV, I II, III, IV, I IV, II, III, I

You want to study a biomolecule in the laboratory. You have ordered the synthetic gene from a company and want to check that it has been introduced into your model organism. What is the proper order of experiments below for doing this? I. Sanger sequencing II. Transformation III. Restriction digest IV. DNA purification from a single colony I, II, III, IV I, IV, II, III III, II, IV, I II, III, IV, I IV, II, III, I

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 2TYK

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Statement 1: Restriction enzymes are sequence-specific, which means that they only recognize a short yet unique sequence and only cleave the DNA in that paticular restriction site. Statement 2: The restriction enzyme used to cut the foreign DNA must be different from the restriction enzyme used to cut the restriction site of a plasmid. O Both statements are true. Both statements are false. Statement 1 is true. Statement 2 is false. Statement 1 is false. Statement 2 is true.
DNA scissors used in genetic engineering applications are called Endo nucleases Restriction enzymes Exo nucleases O DNases
The other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double stranded
Match items in Column A with most related ones in B. Write the letters on the space provided.
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_1. Bacterial proteins that have the ability to cut both strands of the DNA molecule at certain points 2. Contain foreign DNA 3. Is made by connecting segments of DNA from different sources _ 4. General term for a carrier used to transfer a foreign DNA fragment into a host cell 5. A small ring of DŇA found in a bacterial cell 6. The procedure for cleaving DNA from an organism into small segments, and inserting the f. genetic engineering or segments into another organism a. recombinant DNA b. vector c. restriction enzymes d. plasmid e. transgenic organisms recombinant DNA technology
(a) Describe the actions with reason that should be taken under the following situations: (i) An unlabelled collection tube with a requisition for a V Leiden test is received in the laboratory. (ii) After PCR, the amplification control has failed to yield a product. (iii) DNA is stored overnight in a refrigerator set at 8oC but reads 14 oC. (iv) An isolated DNA sample is to be stored for at least 6 months. (v) The expiration date on a reagent has passed. (b) Explain briefly about genetic testing for new-born, including the importance of genetic testing and the disorder that genetic testing can detect.
Dr. Doom has a DNA sequence of 2000 bp. Enzyme X and Enzyme Y restriction sites and their positions are indicated here. Recall that Enzyme X is methylation sensitive, while Enzyme Y is not. 200 O 100 1800 2000 bp 1000 1500 Enzyme Y Enzyme Y Enzyme X Enzyme Y Enzyme X If this gene is methylated, which of the following combinations of DNA fragments would you expect from this restriction digest? O 5 bands = 1O bp, 200 bp, 1000 bp. 1500 bp, and 1800 bp O 3 bands = 200 bp, 500 bp, and 1300 bp %3D O No bands = DNA will be completely digested %3D O 4 bands = 100 bp. 200 bp, 800 bp, and 900 bp
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The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. 1. T7 RNA polymerase 2. Reverse transcriptase 3. EcoRI