**Title: Proper Order of Experiments to Verify Gene Introduction in a Model Organism****Description:**When studying a biomolecule in the laboratory and ordering a synthetic gene, it's crucial to verify its introduction into your model organism. The experiments should be conducted in the following order to ensure accuracy:1. **Restriction Digest (III)** - Begin by using restriction enzymes to verify the presence of the gene by cutting the DNA at specific sites, allowing confirmation of correct gene insertion.2. **Transformation (II)** - Introduce the synthetic gene into the host organism's cells, typically using methods such as heat shock or electroporation for bacterial cells.3. **DNA Purification from a Single Colony (IV)** - Isolate and purify DNA from a single transformed colony to prepare it for sequencing analysis.4. **Sanger Sequencing (I)** - Perform Sanger sequencing to determine the exact nucleotide sequence of the inserted gene, confirming its correct integration and sequence accuracy.**Highlighted Correct Order:**- The order highlighted in green indicates the proper sequence: **III, II, IV, I**This sequence ensures that the gene is properly introduced and accurately verified within the model organism.

The process by which a specific gene sequence of interest is ligated and then the newly synthesized…

Answered: You want to study a biomolecule in the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Select all the statements that provide evidence that DNA is the genetic material. Check All That Apply 1. By weight, a eukaryotic chromosome contains more protein than DNA. 2. Treatment of purified DNA with a DNA-degrading enzyme destroyed the ability of S bacteria to transform R bacteria. 3. The R mutant form of S. pneumonia does not cause infection in mice. 4. Radioactively-labeled DNA remained in bacterial cells after phage T2 infection. 5. Proteins are more complex than DNA because they are composed of 20 different amino acids. Pls help asap!! I am so confused
Please explain right answer
3. Match the following biotech tools with their applications. Some of those tools may have more than one applications. • A. Gene cloning and Recombinant DNA • B. Restriction enzyme • C. Radioactive nucleic acid probe • D. Reverse transcriptase • E. Ti plasmid. • F. Gene therapy • G. PCR • H. Gel electrophoresis • I. single nucleotide polymorphism (SNP) and Restriction fragment length polymorphism (RFLP) • J. Genomic 1. Crime scenes and paternity 2. Making copies of DNA sequence 3. GMO 4. introduce new genes into plant cells 5. alteration of an individual's DNA to treat disease. 6. Cutting DNA at target sequence 7. Detect the presence of specific gene 8. produce a DNA strand from MRNA. 9. separate DNA molecules based on size 10. methods to get the DNA fingerprint of different individual
Dr. Doom has a DNA sequence of 2000 bp. Enzyme X and Enzyme Y restriction sites and their positions are indicated here. Recall that Enzyme X is methylation sensitive, while Enzyme Y is not. 200 O 100 1800 2000 bp 1000 1500 Enzyme Y Enzyme Y Enzyme X Enzyme Y Enzyme X If this gene is methylated, which of the following combinations of DNA fragments would you expect from this restriction digest? O 5 bands = 1O bp, 200 bp, 1000 bp. 1500 bp, and 1800 bp O 3 bands = 200 bp, 500 bp, and 1300 bp %3D O No bands = DNA will be completely digested %3D O 4 bands = 100 bp. 200 bp, 800 bp, and 900 bp
The following statements are true about common gene cloning procedures except: -DNA plasmids can help move around genes into other cells - Restriction enzymes are very important for cutting up and linking chunks of DNA -We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested - DNA plasmids are chunks of chromosomal DNA used for cloning
OF intorcat A Intorest gone in a gene "gun," a bacterial material into the genetic material inserts itself into the chromosomes of the host plant. Engineers must also insert a "promoter" gene from a virus as part of the package to make the inserted gene express itself. . This process alone, involving a gene gun or a similar technique, and a promoter, is profoundly different from conventional breeding, even if the primary goal is only to insert genetic material from the same species (Hansen, 2000). host plant cells. Activity 4: Desirable Traits Directions: Study the plants and animals below that have desirable or enhanced traits. Explain how each of the characteristics was introduced or developed (i.e., classical breeding or recombinant DNA technology). MODIFYING TECHNIQUE (Classical breeding/ Recombinant DNA technology) REASON ENHANCED TRAIT 1. Kobe / Wagyu Beef (Beef with good fat distribution)
The options are: 1. a,b,c,d,f and e 2. c,b, a, e, d and f 3. c, a, b, f, d, and e b, c,e, d, a and f
5. Watch the video: https://www.youtube.com/watch?v=ml0Fo9kaWqo&t=38s. Based on the information in this video and material covered in class answer the following questions: A. In a Sanger sequencing-based PCR reaction, how many primers are added? B. In a Sanger sequencing-based PCR reaction, which molecules are more common: DNTPS or fluorescent terminators? C. What is a typical length of sequence that can be acquired using traditional (Sanger) sequencing? D. The results from sequencing one stretch of DNA is called a "read". How many reads can be obtained from a run on a 384-well plate Sanger sequencing instrument vs. a NovaSeg instrument? E. How many gjgabases of sequences can be obtained on the Sanger vs. NovaSea instruments? Regarding F. and G., the adaptor sequences have two parts: primer binding sites and capture sequences. F. Once the (denatured) DNA has been loaded into the flow cell, which adaptor sequence part is hybridized to other single-stranded DNA first? G. How many copies…
40 When Cas9 cuts DNA, it is a Question Listen DNA 4 Listen Protein When a spacer binds to it's target DNA it is a Questic RNA 40 Protein Question 13 RNA 41 Listen Question. DNA The larger the DNA fragment, the faster it moves through a gel a) True b) False Listen DNA moves toward the positive charged end of a gel a) True b) False Listen You have 200 copies of a DNA sequence. You perform 10 rounds of PCR. How many copies of the DNA sequence will you have? Just write the number. Do not include any commas or blanks.
What is the final volume of the individual PCR reactions we are making?
Arrange the following steps in the PCR experiment 4 Add samples and reagents Extend Anneal Design primers Denature How much template DNA is added in the PCR experiment? 1 nanogram 1 milligram 1 microgram 0.1 grams
1. produces multiple identical copies of a gene [ Choose ] a. DNA ligase b. gene cloning c. gel electrophoresis d.restriction enzymes

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