The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m = b = RNA (µg) 0.00 10.67 21.34 32.01 42.68 mass= Absorbance at 260 nm 0.224 0.512 0.769 1.145 1.465 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.636. μg-1 µg
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m = b = RNA (µg) 0.00 10.67 21.34 32.01 42.68 mass= Absorbance at 260 nm 0.224 0.512 0.769 1.145 1.465 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.636. μg-1 µg
Chemistry & Chemical Reactivity
10th Edition
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions
Section: Chapter Questions
Problem 77PS
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Question
![The data in the table are used to create a calibration curve for the determination of RNA from
its absorbance at 260 nm.
Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the
best straight line fit to the calibration curve. Do not subtract the blank reading when creating
the calibration curve.
m =
b =
RNA
(µg)
0.00
10.67
21.34
32.01
42.68
mass=
Absorbance at 260
nm
0.224
0.512
0.769
1.145
1.465
Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of
0.636.
µg
µg](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff6b731bf-263a-45d3-a264-a5330485f8e3%2Fdd65f696-a557-45ff-93c8-f83db26ebd4f%2Fjbcl3gx_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The data in the table are used to create a calibration curve for the determination of RNA from
its absorbance at 260 nm.
Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the
best straight line fit to the calibration curve. Do not subtract the blank reading when creating
the calibration curve.
m =
b =
RNA
(µg)
0.00
10.67
21.34
32.01
42.68
mass=
Absorbance at 260
nm
0.224
0.512
0.769
1.145
1.465
Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of
0.636.
µg
µg
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