The authors have used a chiral HPLC column (separating phase) to analyze compounds 4 and 5. Briefly describe a) the way a chiral HPLC column works to separate natural products of the Actinopyrone family and b) briefly comment on the general applicability of chiral HPLC columns in natural products research.

Q: What is this formula and how is it derived?? This was used in an experiment related to the screening…

A: Various analytical techniques are used to examine the amounts of carotenoids in biological…

Q: Serum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If…

A: Dyslipoproteinemia is also known as dyslipidemia, which means the abnormal concentration of…

Q: Provide differences between solution-phase peptide synthesis and solid phase synthesis in terms of:…

A: Now a days small peptide is synthesized and tested its drug property to treat a complex disease or…

Q: What causes the DNA to migrate from the wells toward the positive electrode? a) DNA is positively…

A: ANSWER;- b) DNA is negatively charged, and so was attracted to the positive electrode Explain;-  DNA…

Q: This is remdesivir, one of the drugs being used in the treatment of COVID-19. Label the indicated…

A: A chiral center is an atom that has a tetrahedron configuration and has four different ligands…

Q: b) Consider the following experimental results: Total hydrolysis of a nonapeptide gave:…

A: Proteins or peptides are made up of twenty standard amino acids that are attached together via…

Q: Define the term sulfasalazine?

A: Rheumatic diseases are autoimmune diseases which causes widespread inflammation in the body and a…

Q: The Cavity Ring-Down Spectroscopy (CRDS) technique offers greater  sensitivity compared to other…

A:

Q: For whicj of the following problems would ICP-MS be appropriate. Explain why a) For the…

A: ICP-MS (Inductively Coupled Plasma Mass Spectrometry) is an analytical technique that can determine…

Q: Propose a scheme to separate and isolate a mixture containing 4-aminobenzoate,…

A: Extraction is a particularly useful way of separating organic compounds if one molecule in the…

Q: Explain in details how you could separate a mixture of three amino acids Arg, Lys, and Cys using…

A: In order to separate Arginine , lysine and cysteine using ion-exchange chromatography we will use…

Q: What reagents promote cross-linking between acrylamide and bisacrylamide to form polyacrylamide gel?

A: Polyacrylamide gels are used for the separation of biomolecules. The polyacrylamide gels are…

Q: xplain why each of the following measurements is or is not the result of a Bernoulli trial: (a) The…

A: Bernoulli's trial is generally an experiment that leads to two outcomes for samples are success and…

Q: The most commonly used biochemical reducing agent is: a) NADH   b)  NADPH   c)  FADH2    d)  LiALH4…

A: The most commonly used biochemical reducing agent is NADH. NADH is a coenzyme that functions as a…

Q: Which of the following reagents can be used in the reaction in the photo? A.) pyridinium…

A: The oxidation of secondary alcohol to ketones is an important oxidation reaction in organic…

Q: As a part of a clinical study, a pharmacist is asked to prepare a modification of a standard 22 g…

A:

Q: Answer the following questions: 3) Provide some possible errors% in the experiment of synthesis of…

A: Cyclohexene- within the presence of a powerful acid, alcohol are often dehydrated to make an alkene.…

Q: a) b) c) Draw the structure of the following gold complex which is used to treat rheumatoid…

A: Rheumatoid arthritis (RA) is an autoimmune disease that results in a chronic, systemic inflammatory…

Q: An ion-exchange chromatographic separation is performed using a diethyl-aminoethyl- (DEAE)-sepharose…

A: Proteins are composed of twenty standard amino acids attached together via peptide bonds. These…

Q: Ceruloplasmin is a blue-colored monomeric oxidase found in mamma- lian blood plasma. It contains…

A: Ceruloplasmin is a copper containing globulin which helps in the transport of copper in the…

Q: Prepare a standard tube that contains 0.2 mL of 2.5 mM lysine. To this tube, add 0.1 mL of the…

A: Final concentration can be calculated from the formula C1V1=C2V2 Where C1= initial concentration V1=…

Q: What are other chromatographic techniques that can be used for separating non-polar biomolecules…

A: Introduction: Chromatography is a method for separating a mixture in the laboratory. The mixture is…

Q: ch of these heterocycli drugs is likely to be the least soluble in water? Use parameter to decide.…

A: To assess the water solubility of heterocyclic drugs, we can use the fsp3 parameter. This parameter…

Q: 3. Determine the primary structure of pentadecapeptide X, based on the following results. A).…

A: Determination of protein primary structure is done by degradation with different enzymes which have…

Q: Please provide a separation solution of combining two types of chromatography to isolate the protein…

A: Here, we can see that two proteins (protein A and protein C) are non-polar in nature while the other…

Q: A solution containing egg albumin (pl=4.6), B-lactoglobulin (pl=5.2), and chymotrypsinogen (pl=9.5)…

A: Column chromatography is a technique used for the separation of solutes present in the sample based…

Q: Propose a mechanism for the conversion of 2,4,6-trimethylphenol to compound A.

A: SN2 reaction: The alcohols react with acids like hydrochloric acid or hydrobromic acid, the bromine…

Q: What volume of water is needed to make the following primer stocks solutions? Primer 1. 25 nM…

A: DNA Replication is the process by which one double-stranded DNA molecules gets replicated to produce…

Q: Compounds I, II, and III are in the following biochemical pathway: precursor compound I enzyme A…

A: Cell biology is considered as the study of cells, their structure, and their functions. The cells…

Q: How to efficiently obtain enantiomerically pure products? - Compare at least two techniques for…

A: Methods to efficiently obtain enantiomerically pure products are: 1.Gas chromatography (GC), 2.High…

Q: Cimetidine is used in the treatment and management of gastric ulcers and gastroesophageal reflux…

A: Desired dose of Cimetidine = 37.5mg/hourDose in hand = 6mg/mlQuantity required = Desired dose/Dose…

Q: Penicillin is hydrolyzed and thereby rendered inactive by penicillinase, an enzyme present in some…

A: Hi! As you have posted multiple subparts and have not mentioned which is to be answered, we are…

Q: Arrange the following in order of increasing Rf on thin-layer chromatography: acetic acid,…

A: Thin-layer chromatography is a method of separating non-volatile mixtures. Thin-layer chromatography…

Q: Enzyme X has a molecular weight of 48,000. It converts substrate Z into product Y. Z absorbs at 340…

A:

Q: 25) Although the UV280 extinction coefficient of BSA is well known, why was the BCA assay employed…

A: Protein assays normally used to standards to quantify quality of other protein, by comparing unknown…

Q: need to dissolve cholesterol in Chloroform in the Liebermann-Burchard Test

A: The reaction of acetic anhydride in chloroform with unsaturated steroids (e.g. cholesterol) in the…

Q: What does it mean for chitosan to have high bioadhesive properties? In which areas is this feature…

A: Chitosan is a polysaccharide unit and one of the ingredients produced from the chitin of marine…

Q: An amino acid mixture consisting of lysine,leucine, and glutamic acid is to be separated by…

A: In reverse-phase HPLC with a non polar stationary phase and a polar solvent as the mobile phase at…

Q: What is the purpose of Lugol's solution?  What is the use of Benedict's solution?

A: Jean Lugol, a physician from France was the first to introduce Lugol's solution in the year 1829. It…

Q: Will the synthesis of 9,10-dihydroanthracene-9,10-α,β-succinic anhydride via Diels-Alder reaction…

A: In organic chemistry, chemical reactions involve the making and breaking of chemical bonds.  On the…

Q: If a pharmacist added each of the following to 22-g packages of 2% mupirocin ointment, what would be…

A: Mupirocin is an antibiotic that prevents bacteria from growing on your skin. Mupirocin topical is…

Q: ou resuspend 1 ml of of a purified chlorophyll sample in 10 ml of 80% acetone, and determine that…

A: The Beer-Lambert Law (also called Beer’s Law) is used to determine the concentration of unknown…

Q: Name four important amines that are producing during cadaver degeneration. Explain each.

A:

Q: Briefly explain the mode of action of taxanes in relation to the experiment we carried out. What…

A: Cell divison is the process of replication of the cellular genetic material by a series of events…

Q: A decapeptide which could resist burns was isolated from an endemic plant from Mt. Mayon. Deduce the…

A: The cleavage sites of the different enzymes and chemical compounds used for the determination of the…

Q: biologically derived hydroxyapatite? b) How do you define porous and dense hydroxyapatite? Ple…

A: Hydroxyapatite is a hexagonal crystal type of the mineral calcium apatite, which contains calcium,…