An amino acid mixture consisting of lysine,leucine, and glutamic acid is to be separated by ion-exchangechromatography, using a cation-exchange resin at pH 3.5, with theeluting buffer at the same pH. Which of these amino acids will beeluted from the column first? Will any other treatment be neededto elute one of these amino acids from the column?

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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An amino acid mixture consisting of lysine,
leucine, and glutamic acid is to be separated by ion-exchange
chromatography, using a cation-exchange resin at pH 3.5, with the
eluting buffer at the same pH. Which of these amino acids will be
eluted from the column first? Will any other treatment be needed
to elute one of these amino acids from the column?

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