For production of penicillin (C16H18O4N2S) using Penicillium mold, glucose (C6H1206) is used as a substrate and phenylacetic acid (C8H8O2) is added as a precursor. The stoichiometry for overall synthesis is: 1.67 C6H1206 + 2NH3 + 0.502+ H2SO4 + C3H3O2 → C16H1804N2S + 2CO2 + 9H20 a) What is the maximum theoretical yield of penicillin from glucose in g/g? b) When results from a particular penicillin fermentationvere analyzed, it was found that 24% of the glucose had been used for growth, 70% for cell maintenance activities (such as membrane transport and macromolecule turnover) and only 6% for penicillin synthesis. Calculate the yield of penicillin from glucose in g/g under these conditions. c) Batch fermentation under the conditions described in part b) is carried out in a 100-liter tank. Remember that only 6% of the glucose is used for penicillin synthesis. Initially, the tank is filled with nutrient medium containing 50 g Lglucose and 4 g L phenylacetic acid. If the reaction is stopped when the glucose concentration is 5.5 g L1, determine
For production of penicillin (C16H18O4N2S) using Penicillium mold, glucose (C6H1206) is used as a substrate and phenylacetic acid (C8H8O2) is added as a precursor. The stoichiometry for overall synthesis is: 1.67 C6H1206 + 2NH3 + 0.502+ H2SO4 + C3H3O2 → C16H1804N2S + 2CO2 + 9H20 a) What is the maximum theoretical yield of penicillin from glucose in g/g? b) When results from a particular penicillin fermentationvere analyzed, it was found that 24% of the glucose had been used for growth, 70% for cell maintenance activities (such as membrane transport and macromolecule turnover) and only 6% for penicillin synthesis. Calculate the yield of penicillin from glucose in g/g under these conditions. c) Batch fermentation under the conditions described in part b) is carried out in a 100-liter tank. Remember that only 6% of the glucose is used for penicillin synthesis. Initially, the tank is filled with nutrient medium containing 50 g Lglucose and 4 g L phenylacetic acid. If the reaction is stopped when the glucose concentration is 5.5 g L1, determine
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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