For production of penicillin (C16H18O4N2S) using Penicillium mold, glucose (C6H1206) is used as a substrate and phenylacetic acid (C8H8O2) is added as a precursor. The stoichiometry for overall synthesis is: 1.67 C6H1206 + 2NH3 + 0.502+ H2SO4 + C3H3O2 → C16H1804N2S + 2CO2 + 9H20 a) What is the maximum theoretical yield of penicillin from glucose in g/g? b) When results from a particular penicillin fermentationvere analyzed, it was found that 24% of the glucose had been used for growth, 70% for cell maintenance activities (such as membrane transport and macromolecule turnover) and only 6% for penicillin synthesis. Calculate the yield of penicillin from glucose in g/g under these conditions. c) Batch fermentation under the conditions described in part b) is carried out in a 100-liter tank. Remember that only 6% of the glucose is used for penicillin synthesis. Initially, the tank is filled with nutrient medium containing 50 g Lglucose and 4 g L phenylacetic acid. If the reaction is stopped when the glucose concentration is 5.5 g L1, determine

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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For production of penicillin (C16H18O4N2S) using Penicillium mold, glucose (C6H12O6) is used as a substrate and
phenylacetic acid (C3H8O2) is added as a precursor. The stoichiometry for overall synthesis is:
1.67 C6H1206 + 2NH3 + 0.502+ H2SO4+ C3H8O2 → C16H180,N2S + 2CO2+ 9H2O
a) What is the maximum theoretical yield of penicillin from glucose in g/g?
b) When results from a particular penicillin fermentationvere analyzed, it was found that 24% of the glucose
had been used for growth, 70% for cell maintenance activities (such as membrane transport and
macromolecule turnover) and only 6% for penicillin synthesis. Calculate the yield of penicillin from
glucose in g/g under these conditions.
c) Batch fermentation under the conditions described in part b) is carried out in a 100-liter tank. Remember
that only 6% of the glucose is used for penicillin synthesis. Initially, the tank is filled with nutrient medium
containing 50 gL glucose and 4 g L phenylacetic acid. If the reaction is stopped when the glucose
concentration is 5.5 g L1, determine
Transcribed Image Text:For production of penicillin (C16H18O4N2S) using Penicillium mold, glucose (C6H12O6) is used as a substrate and phenylacetic acid (C3H8O2) is added as a precursor. The stoichiometry for overall synthesis is: 1.67 C6H1206 + 2NH3 + 0.502+ H2SO4+ C3H8O2 → C16H180,N2S + 2CO2+ 9H2O a) What is the maximum theoretical yield of penicillin from glucose in g/g? b) When results from a particular penicillin fermentationvere analyzed, it was found that 24% of the glucose had been used for growth, 70% for cell maintenance activities (such as membrane transport and macromolecule turnover) and only 6% for penicillin synthesis. Calculate the yield of penicillin from glucose in g/g under these conditions. c) Batch fermentation under the conditions described in part b) is carried out in a 100-liter tank. Remember that only 6% of the glucose is used for penicillin synthesis. Initially, the tank is filled with nutrient medium containing 50 gL glucose and 4 g L phenylacetic acid. If the reaction is stopped when the glucose concentration is 5.5 g L1, determine
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