The Ames test uses the reversion rate (His− to His+)to test compounds for mutagenicity.a. Is it possible that a known mutagen, like proflavin,would be unable to revert a particular His− mutantused in the Ames test? How do you think that theAmes test is designed to deal with this issue?b. Can you think of a way to use forward mutation(His+ to His−) to test a compound for mutagenicity? (Hint: Consider using the replica plating technique in Fig. 7.6.)c. Given that the rate of forward mutation is so muchhigher than the rate of reversion, why does the Amestest use the reversion rate to test for mutagenicity?
The Ames test uses the reversion rate (His− to His+)to test compounds for mutagenicity.a. Is it possible that a known mutagen, like proflavin,would be unable to revert a particular His− mutantused in the Ames test? How do you think that theAmes test is designed to deal with this issue?b. Can you think of a way to use forward mutation(His+ to His−) to test a compound for mutagenicity? (Hint: Consider using the replica plating technique in Fig. 7.6.)c. Given that the rate of forward mutation is so muchhigher than the rate of reversion, why does the Amestest use the reversion rate to test for mutagenicity?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The Ames test uses the reversion rate (His− to His+)
to test compounds for mutagenicity.
a. Is it possible that a known mutagen, like proflavin,
would be unable to revert a particular His− mutant
used in the Ames test? How do you think that the
Ames test is designed to deal with this issue?
b. Can you think of a way to use forward mutation
(His+ to His−) to test a compound for mutagenicity? (Hint: Consider using the replica plating technique in Fig. 7.6.)
c. Given that the rate of forward mutation is so much
higher than the rate of reversion, why does the Ames
test use the reversion rate to test for mutagenicity?
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