Sb Table 1: SDS samples Vol. protein Lane Sample sample (ul) 1 Blank 0 Vol. 2x Red Sample Buffer (µL) 20 23456700 Protein Ladder 4 16 Lysate 10 10 Flowthrough 10 10 Wash 10 10 E1 10 10 E2 10 10 8 E3 10 10 9 Dialyzed VRK1 10 10 10 LDH (standard) 10 10 11 Blank 0 20

Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter22: Proteins
Section: Chapter Questions
Problem 22.68P
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Please analze my gel electrophoresis column of the VRK1 kinase (MW: 39.71 kDa). 

Attached is the following image for the order of column wells and my gel.

 

Sb
Transcribed Image Text:Sb
Table 1: SDS samples
Vol. protein
Lane
Sample
sample (ul)
1
Blank
0
Vol. 2x Red Sample
Buffer (µL)
20
23456700
Protein Ladder
4
16
Lysate
10
10
Flowthrough
10
10
Wash
10
10
E1
10
10
E2
10
10
8
E3
10
10
9
Dialyzed VRK1
10
10
10
LDH (standard)
10
10
11
Blank
0
20
Transcribed Image Text:Table 1: SDS samples Vol. protein Lane Sample sample (ul) 1 Blank 0 Vol. 2x Red Sample Buffer (µL) 20 23456700 Protein Ladder 4 16 Lysate 10 10 Flowthrough 10 10 Wash 10 10 E1 10 10 E2 10 10 8 E3 10 10 9 Dialyzed VRK1 10 10 10 LDH (standard) 10 10 11 Blank 0 20
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