Sketch 1/v v.s. 1/[s] for enzyme 1 and enzyme 2 where enzyme 1 has same Vmax as enzyme 2 but has larger Km.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Sketch 1/v v.s. 1/[s] for enzyme 1 and enzyme 2 where enzyme 1 has same Vmax as enzyme 2 but has larger Km.
Enzymes are bio-catalyst that participate in biochemical process and they are highly specific in their action. They help to speed up the reaction rate. They have active site where substrate binds and catalysis of reaction occurs. Enzymes are mostly proteins but there is an exception of ribozymes which are not protein but catalytic RNA molecules.
Lineweaver-Burke Plot is also known as double reciprocal plot. In such graph, x-axis has 1/[S] and y-axis has 1/V values.
[S] means substrate concentration, V is velocity of the reaction, Vmax is the maximum velocity attained by the reaction & Km indicates the Michaelis constant.
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