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What is cross linking? Write down its advantages and disadvantages.

What is cross linking? Write down its advantages and disadvantages.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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a) What is cross linking? Write down its advantages and disadvantages.
4:07 54 Edit Advantages : the binding force between enzyme and carrier is so strong that no leakage of the enzymes occurs, even in the presence of substrate or solution of high ionic strength. Cross Linking • Cross linking involves intermolecular cross linking of enzyme molecules in the presence/absence of solid support. The method produces a 3 dimensional cross linked enzyme aggregate (insoluble in water) by means of a multifunctional reagent that links covalently to the enzyme molecules. Advantages of cross linking:- 1. Very little desorption(enzyme strongly bound) Higher stability (i.e. ph, ionic & substrate concentration) Disadvantages of cross linking:- 1. Cross linking may cause significant changes in the active site. 2. Not cost effective. A ton Covale SSE ENZYME IMMOBILZATION DO Tools Play Share Edit on PC
Transcribed Image Text:4:07 54 Edit Advantages : the binding force between enzyme and carrier is so strong that no leakage of the enzymes occurs, even in the presence of substrate or solution of high ionic strength. Cross Linking • Cross linking involves intermolecular cross linking of enzyme molecules in the presence/absence of solid support. The method produces a 3 dimensional cross linked enzyme aggregate (insoluble in water) by means of a multifunctional reagent that links covalently to the enzyme molecules. Advantages of cross linking:- 1. Very little desorption(enzyme strongly bound) Higher stability (i.e. ph, ionic & substrate concentration) Disadvantages of cross linking:- 1. Cross linking may cause significant changes in the active site. 2. Not cost effective. A ton Covale SSE ENZYME IMMOBILZATION DO Tools Play Share Edit on PC
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