Shown in Appendix 1 is an example of a general-purpose cloning vector.  A typical plasmid/vector map can indicate the size of the plasmid, the components found on the plasmid and the locations of commonly used restriction sites. The number represents the position of the 5’ most- base on the top strand within the recognition sequence.  Enzymes that only cut the plasmid at one location (unique) are shown in BOLD.  A vector map is often referred to as a restriction map as it allows one to predict the size of fragments obtained when ‘cutting’ the plasmid with chosen restriction enzymes.     Q1. Identify the following components of the vector and describe what their role is    Origin of Replication (ORI)     AmpR   MCS   Q2. What does the arrow represent?        Q3. Genetic engineering of bacterial cells can result in the production of a human protein of interest by inserting the gene of interest (example human insulin gene) into a plasmid (expression vector). Upon introduction of the recombinant plasmid into bacteria, transcription and translation of human gene will occur. If you introduced the insulin gene into the MCS of the vector shown in Appendix 1 using appropriate enzymes, this recombinant plasmid WILL NOT result in protein production in a bacterial cell. Explain Why? What modification will you need to make to the plasmid to convert the general-purpose vector into an expression vector?