QUESTION 7 "ou are trying to use qPCR to compare gene expression of nitrous oxide reductase (nosz) in a Pseudomonas sample treated under various com e-delta deltact method, about how much higher (or lower) is the expression of nitrous oxide reductase (nosZ) in the experimentally treated sa untreated sample? nosz Ct values for untreated sample (control conditions) Internal housekeeping nosz Ct values for Internal housekeeping gene Ct values for untreated sample (control conditions) experimental gene Ct values for treatment sample experimental treatment sample Replicate 1 21.3 20.0 19.8 20.9 Replicate 2 21.5 20.1 19.7 21.0 Replicate 3 21.4 20.3 19.5 20.7 Replicate 4 21.6 20.2 19.4 21.1 O A. -2 times higher O B. -100 times higher O C. about 20% as much as the control O D.-3.5 times higher O E. -12 times higher O F. -6 times higher than the control

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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QUESTION 7
You are trying to use qPCR to compare gene expression of nitrous oxide reductase (nosZ) in a Pseudomonas sample treated under various conditions. Using the
2-delta deltact method, about how much higher (or lower) is the expression of nitrous oxide reductase (nosZ) in the experimentally treated sample verses the
untreated sample?
nosZ Ct values for
untreated sample
(control conditions)
Internal housekeeping
nosz Ct values for Internal housekeeping
gene Ct values for
untreated sample
(control conditions)
gene Ct values for
treatment sample experimental
treatment sample
experimental
Replicate 1
21.3
20.0
19.8
20.9
Replicate 2
21.5
20.1
19.7
21.0
Replicate 3
21.4
20.3
19.5
20.7
Replicate 4
21.6
20.2
19.4
21.1
O A. -2 times higher
B. -100 times higher
C. about 20% as much as the control
O D.-3.5 times higher
O E. -12 times higher
O F. -6 times higher than the control
Transcribed Image Text:QUESTION 7 You are trying to use qPCR to compare gene expression of nitrous oxide reductase (nosZ) in a Pseudomonas sample treated under various conditions. Using the 2-delta deltact method, about how much higher (or lower) is the expression of nitrous oxide reductase (nosZ) in the experimentally treated sample verses the untreated sample? nosZ Ct values for untreated sample (control conditions) Internal housekeeping nosz Ct values for Internal housekeeping gene Ct values for untreated sample (control conditions) gene Ct values for treatment sample experimental treatment sample experimental Replicate 1 21.3 20.0 19.8 20.9 Replicate 2 21.5 20.1 19.7 21.0 Replicate 3 21.4 20.3 19.5 20.7 Replicate 4 21.6 20.2 19.4 21.1 O A. -2 times higher B. -100 times higher C. about 20% as much as the control O D.-3.5 times higher O E. -12 times higher O F. -6 times higher than the control
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