QUESTION 56PDGFHILL100PI3KPI3K75150GAP OO GAP771protein Psite12PTP O 1009 O PTPPI3K740, 751GAP7711021PTP1009PLCPLCPLC-y1021When activated by ligand binding, the PDGF (platelet-derived growth factor) receptor becomes phosphorylated on 5 tyrosine residues (left figure). These phosphorylated tyrosines serve as binding sites forproteins that contain SH2 domains (SH2 domains bind phosphorylated Y). These proteins include phospholipase C-gamma (PLC-gamma), a phosphotyrosine phosphatase (PTP), a Ras GTPase-Activating Protein (GAP), and a phosphotidylinositol 3-Kinase (PI3K). PDGF stimulates several changes in the target cell, one of which is DNA synthesis. To determine which effectors of the PDGFreceptor is/are responsible for stimulating DNA synthesis, you construct several mutant forms of the receptor that retain individual or combinations of the phosphorylation sites. You express these in cellsand monitor DNA synthesis. The results are shownthe right figure.Which effector suppresses DNA synthesis?O a. PTPO b. PI3KO c. GAPO d. PLC-gamma

Name: QUESTION 56PDGFHILL100PI3KPI3K75150GAP OO GAP771protein Psite12PTP O
Uploaded: 2024-03-20T06:42:41.000Z
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Description: QUESTION 56PDGFHILL100PI3KPI3K75150GAP OO GAP771protein Psite12PTP O 1009 O PTPPI3K740, 751GAP7711021PTP1009PLCPLCPLC-y1021When activated by ligand binding, the PDGF (platelet-derived growth factor) receptor becomes phosphorylated on 5 tyrosine resi

Genes store genetic information in the form of DNA, which may be converted into functional proteins for carrying out life activities. These genes occasionally undergo "mutations", which alter the instructions for forming the protein, causing the protein to malfunction. Answer: c. GAP Explanation: The first column contains the genes Pi3k, GAP, PTP, and PLC-gamma. When all but Pi3k are altered, the DNA synthesis rate is reduced by just 25%. This suggests that the Pi3k protein is the most crucial in DNA replication.Following that, the lack of all the genes in column 9 reveals the basal replication rate. In column 4, where just PTP is present, we observe a comparable basal level. This suggests that the presence of PTP is insufficient to initiate DNA replication. The same is true for column 3 with only GAP protein.Only PLC-gamma is present in column 5, and the replication rate is the same as in column 2, indicating that PLC-gamma is a significant regulator of DNA replication that works independently of Pi3k. As we can see in column 6, the situation is identical. However, due to the presence of GAP protein in column 7, the rate is low. As a result, GAP may serve as an inhibitor or result in PLC-gamma dephosphorylation.As previously stated in column 8, PTP has no influence on PLC-gamma. As a result, we may conclude that the proteins pi3k and PLC-gamma are the most critical effectors for DNA replication. PLC-gamma is inhibited by the GAP protein.