In order to separate protein X using ion exchange chromatography, I am using a resin with Schiff nitrogens attached (pKa is 6.0), If I wanted to separate protein X, whose pl is 7.0, what is the pH range of the buffer that can be used for ion exchange chromatography with this resin? there is no pH range for this resin and protein X. O any pH above 6.0. ) any pH between 6.0 and 7.0 any pH below 7.0

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Question 26
In order to separate protein X using ion exchange chromatography, I am using a resin
with Schiff nitrogens attached (pKa is 6.0). If I wanted to separate protein X, whose
pl is 7.0, what is the pH range of the buffer that Can be used for ion exchange
chromatography with this resin?
there is no pH range for this resin and protein X.
any pH above 6.0.
any pH between 6.0 and 7.0
any pH below 7.0
Transcribed Image Text:Question 26 In order to separate protein X using ion exchange chromatography, I am using a resin with Schiff nitrogens attached (pKa is 6.0). If I wanted to separate protein X, whose pl is 7.0, what is the pH range of the buffer that Can be used for ion exchange chromatography with this resin? there is no pH range for this resin and protein X. any pH above 6.0. any pH between 6.0 and 7.0 any pH below 7.0
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