You analyze the same mixture of proteins from the problem shown in the image, by anion exchange chromatography at ph 7.0. After the proteins were eluted with increasing concentration of NaCl, the chromatogram shown below was obtained. What protein will correspond to peak number 4? a. Protein ab. Protein ec. Protein dd. Protein be. Protein c You analyzed a mixture of five different proteins (a, b, c, d and e) by two-dimensional (2D) gel electrophoresis and obtained the gel shown below. If youanalyze that same mixture of proteins by gel filtration chromatography, whichprotein would you expect to be eluted first?Isoelectric focusingpH 5pH 10protein bprotein eprotein cprotein dprotein aSDS PAGE electrophoresis

Gel electrophoresis is used to separate molecules based on its size and charge of the proteins.…

Answered: You analyze the same mixture of…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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You analyze the same mixture of proteins from the problem shown in the image, by anion exchange chromatography at ph 7.0. After the proteins were eluted with increasing concentration of NaCl, the chromatogram shown below was obtained. What protein will correspond to peak number 4?

a. Protein a

b. Protein e

c. Protein d

d. Protein b

e. Protein c

You analyzed a mixture of five different proteins (a, b, c, d and e) by two-
dimensional (2D) gel electrophoresis and obtained the gel shown below. If you
analyze that same mixture of proteins by gel filtration chromatography, which
protein would you expect to be eluted first?
Isoelectric focusing
pH 5
pH 10
protein b
protein e
protein c
protein d
protein a
SDS PAGE electrophoresis

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