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You analyze the same mixture of proteins from the problem shown in the image, by anion exchange chromatography at ph 7.0. After the proteins were eluted with increasing concentration of NaCl, the chromatogram shown below was obtained. What protein will correspond to peak number 4? a. Protein a b. Protein e c. Protein d d. Protein b e. Protein c

You analyze the same mixture of proteins from the problem shown in the image, by anion exchange chromatography at ph 7.0. After the proteins were eluted with increasing concentration of NaCl, the chromatogram shown below was obtained. What protein will correspond to peak number 4? a. Protein a b. Protein e c. Protein d d. Protein b e. Protein c

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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You analyze the same mixture of proteins from the problem shown in the image, by anion exchange chromatography at ph 7.0. After the proteins were eluted with increasing concentration of NaCl, the chromatogram shown below was obtained. What protein will correspond to peak number 4?

 

a. Protein a

b. Protein e

c. Protein d

d. Protein b

e. Protein c

 

You analyzed a mixture of five different proteins (a, b, c, d and e) by two- dimensional (2D) gel electrophoresis and obtained the gel shown below. If you analyze that same mixture of proteins by gel filtration chromatography, which protein would you expect to be eluted first? Isoelectric focusing pH 5 pH 10 protein b protein e protein c protein d protein a SDS PAGE electrophoresis
Transcribed Image Text:You analyzed a mixture of five different proteins (a, b, c, d and e) by two- dimensional (2D) gel electrophoresis and obtained the gel shown below. If you analyze that same mixture of proteins by gel filtration chromatography, which protein would you expect to be eluted first? Isoelectric focusing pH 5 pH 10 protein b protein e protein c protein d protein a SDS PAGE electrophoresis
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