pH 5.3 buffer -pH 3.3 buffer- -pH 4.3 buffer- Asp Thr Ala Met His Glu THC Ser lle Leu Tyr Phe NH, Gly Val Lys Arg Pro 120 160 200 240 280 320 330 370 410 450 490 550 590 630 80 40 Effluent (mL)

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Why are buffer solutions of increasingly higher pH used to elute the column that generates the chromatogram

pH 5.3 buffer
-pH 3.3 buffer-
-pH 4.3 buffer-
Asp
Thr
Ala
Met
His
Glu
THC
Ser
lle
Leu
Tyr
Phe
NH,
Gly
Val
Lys
Arg
Pro
120 160 200 240 280 320 330 370 410 450 490 550 590 630
80
40
Effluent (mL)
Transcribed Image Text:pH 5.3 buffer -pH 3.3 buffer- -pH 4.3 buffer- Asp Thr Ala Met His Glu THC Ser lle Leu Tyr Phe NH, Gly Val Lys Arg Pro 120 160 200 240 280 320 330 370 410 450 490 550 590 630 80 40 Effluent (mL)
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